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血管瘤病变型J亚群禽白血病病毒E元件缺失突变体的构建

Construction of an infectious clone of subgroup J Avian leukosis virus with E element deletion
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摘要 为构建血管瘤病变型J亚群禽白血病病毒E元件缺失突变体病毒,本研究利用融合PCR方法将E元件缺失,获得含有缺失性突变体病毒前病毒全基因组的重组质粒pSCAU-HN-△E。将该重组质粒纯化后转染DF-1细胞,并连续传代,最终获得缺失病毒rSCAU-HN-△E。该毒株感染的DF-1细胞可被ALV-J特异性单克隆抗体JE9所识别,并且体外增殖性能稳定。该缺失性病毒的获得为E元件功能的研究奠定了基础。 The Subgroup J avian leukosis virus （ALV-J） strain SCAU-HN06 isolated from commercial layer hens with spontaneous hemangiomas has a complete ＂E＂ element in 3＇UTR. In order to rescue the E element-deleted virus, we constructed the recombinant plasmid pSCAU-HN-△ E that contained the whole ALV-J genome but with E element deleted. The plasmid was transfected into DF-1 cells and the rescued virus was identified by indirect fluorescence antibody test with ALV-J specific monoclonal antibody JE9. The recombinant virus was stable in cell culture during nine passages and had a titre up to 10^3.6 TCID50/0.2 mL.
作者 张贺楠 齐岩 史伟伟 梁艺瑜 刘洪波 张小桃 曹伟胜 廖明
机构地区 华南农业大学兽医学院/农业部动物疫病防控重点开放实验室 农业部动物疫病防控重点开放实验室 中国牧工商(集团)总公司
出处 《中国预防兽医学报》 CAS CSCD 北大核心 2010年第2期94-97,共4页 Chinese Journal of Preventive Veterinary Medicine
基金 国家自然科学基金(30771612) NSFC-广东联合基金(U0831002) 广东省自然科学基金(8151064201000065) 广东省科技计划项目(20090204)
关键词 J亚群禽白血病病毒 血管瘤 E元件 感染性克隆 subgroup J Avian leukosis virus Hemangioma E element infectious clone
分类号 S852.65 [农业科学—基础兽医学]
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参考文献17

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二级参考文献47

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中国预防兽医学报
2010年 第2期

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