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人泡沫病毒介导IL-24对肿瘤细胞的抑制作用 被引量：1

Inhibitive effect of human foamy virus induced IL-24 on cancer cells
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摘要 目的:利用重新构建的人逆转录病毒四载体系统共转染人胚肾细胞获得携带IL-24的复制缺陷型重组人泡沫病毒(HFV-IL24)颗粒,探讨复制缺陷型人泡沫病毒介导IL-24对肿瘤细胞生长分化的抑制作用。方法:重组构建含IL-24的人泡沫病毒载体质粒(pΔΦ-IL24);与辅助质粒共转染人胚肾HEK293T细胞后获取重组病毒颗粒(HFV-IL24);RT-PCR检测目的基因的表达;MTT法检测人宫颈癌HeLa细胞的体外增殖水平,台盼蓝染色计数检测细胞存活率,流式细胞术检测细胞活性和周期变化。结果:成功构建含IL-24的人泡沫病毒载体(pΔΦ-IL24);收获得到复制缺陷型重组人泡沫病毒颗粒(HFV-IL24);感染后的HeLa细胞显示出明显的生长抑制现象(P<0.01)并呈现周期变化和较高的凋亡(死亡)率。结论:所构建的重组病毒载体pΔΦ-IL24及由此而产生的重组人泡沫病毒颗粒(HFV-IL24)的功能性检测加深对HFV和抗癌基因IL-24的了解,为泡沫病毒载体的应用和IL-24抗肿瘤的基因治疗提供了研究方向和理论依据。 AIM：To construct replication-defective HFV-IL24 virus vector and to investigate its inhibitive effect on cancer cells after infected or transfected by this vector.METHODS：pΔΦ-IL24 was constructed and was co-transfected with helper-plasmids into HEK 293T cells.Recombinant HFV-IL24 vector was extracted to infect HeLa cells and the inhibitive effect of IL-24 on cancer cells was examined by RT-PCR,MTT,cell counting and FCM methods.RESULTS：pΔΦ-IL24 plasmid and HFV-IL24 particles were successfully constructed.HeLa cells exhibited obvious growth inhibition,cell cycle variation and visible apoptosis（mortality） after infected or transfected by HFV-IL24 or pΔΦ-IL24.CONCLUSION：The results of the study provide some evidence for human foamy virus used as an effective gene transfer tool and for cancer gene therapy with HFV-IL24.
作者 陈思翀 姚辰 陈芳 刘万红 陶卫萍 李文鑫
机构地区 武汉大学生命科学学院 中国科学院西双版纳热带植物园 武汉大学基础医学院 武汉大学人民医院肿瘤科
出处 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2010年第2期121-124,共4页 Chinese Journal of Cellular and Molecular Immunology
基金 国家大学生创新训练项目资助(2006-061048619)
关键词 人泡沫病毒 白细胞介素24 病毒载体 基因治疗 human foamy virus interleukin-24 virus vector gene therapy
分类号 R392.11 [医药卫生—免疫学]
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参考文献10

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同被引文献28

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细胞与分子免疫学杂志
2010年 第2期

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