摘要
沙眼衣原体(Chlamydia trachomatis,Ct),可导致沙眼、感染性致盲,且是引起泌尿生殖道感染等性传播疾病的主要病原体之一。本文利用常温常规电镜制样方法和电子断层成像技术,体外对临床上分离得到的沙眼衣原体进行了研究。研究发现,此种衣原体在发育过程中,胞质发生了一系列的变化,首先RB胞质浓缩变小形成具有类核的IB,随后发育成高度致密且体积较小的EB。伴随此过程,膜也发生了变化:首先RB的膜易皱缩,EB的膜则较坚固;其次RB发育成EB时,多余外膜会形成囊泡;此外,其外膜结构随着RB发育为EB而变厚并更稳定。
As a significant pathogen,Chlamydia trachomatis causes genital and ocular infections in humans：the genital serovars and the ocular serovars of Chlamydia trachomatis cause the sexually transmitted disease and blinding trachoma respectively.The conventional Bio-TEM technologies together with the electron tomography were applied to study a strain of clinical isolated Chlamydia trachomatis.The results demonstrated that part of the cytoplasm of RBs gradually shrank and formed IBs with condensed karyoid during the development process,and it became extremely dense in EBs.The membranes also changed during following processes：at first,the membranes of RBs were easy to be broken and shrunk,while the membranes of EBs were rigid;and then,when RBs developed into EBs,the superfluous outer membranes intended to form vesicles;finally,the membranes were becoming thicker and developed into EBs.
出处
《电子显微学报》
CAS
CSCD
北大核心
2009年第6期543-547,共5页
Journal of Chinese Electron Microscopy Society
基金
国家人才基金资助项目(No.J0730638)
关键词
沙眼衣原体
电子断层成像技术
三维结构
Chlamydia trachomatis
electron tomography
three dimensional structure
