摘要
目的:探讨绒毛细胞染色体制备的最佳方法及其临床意义。方法:112例早期绒毛细胞分别采用直接法或培养法制备染色体,并进行染色体核型分析。结果:在112例绒毛细胞染色体核型中,绒毛染色体异常38例(33.9%);常见异常核型为染色体数目异常26例,占异常核型68.4%,其中三体16例,占异常核型42.1%,单体和三倍体各5例,各占异常核型13.2%;嵌合体2例,占异常核型5.3%;结构异常10例,占异常核型26.3%。结论:先兆流产、自然流产与染色体异常的发生有直接关系,染色体检查对查找自然流产的原因和选择合理的治疗有重要的临床意义。绒毛细胞染色体检查培养法优于直接制备法,可在临床中广泛推广。
Objective： To explore an optimal method of chromosomal preparation of chorionic villus cells and its clinical significance. Methods： Chromosomal preparation of chorionic villus cells in 112 cases was conducted with direct -preparation method or cultivation method, respectively, then karyotype analysis was carried out. Results： 38 cases were diagnosed as chromosomal abnormality, the incidence was 33.9%. The common abnormal karyotype was chromosome number abnormality, accounting for 68.4% （26 cases ）, including 16 cases of trisomy syndrome （42. 1% ）, 5 cases of monosomy syndrome （ 13.2% ） and 5 cases of triploidy syndrome （ 13.2% ） ; the other chromosomal abnormalities included chimera （2 cases, 5.3% ） and structural abnormality （10 cases, 26. 3% ） . Conclusion： Threatened abortion and spontaneous abortion are related to occurrence of chromosomal abnormality directly, chromosomal examination plays an important role in seeking predisposing factors of spontaneous abortion and choosing appropriate therapy, cultivation method is superior to direct - prepa- ration method in chromosomal examination of chorionic villus cells, which deserves clinical expansion.
出处
《中国妇幼保健》
CAS
北大核心
2010年第6期813-815,共3页
Maternal and Child Health Care of China
基金
福建省科技厅重大计划项目〔2007Y0011〕
关键词
绒毛组织
染色体分析
直接制备法
细胞培养
Chorionic villus
Karyotype analysis
Direct - preparation method
Cell cultivation
