摘要
目的:探讨黄芪多糖(Astragalus Polysaccharide,APS)对哮喘小鼠特异性免疫治疗(Specific Immune Therapy,SIT)的影响及其可能的作用机制。方法:90只4～6周龄SPF级BALB/c小鼠,随机平均分为空白对照组、哮喘模型组、SIT治疗组、APS治疗组、APS和SIT单纯联合治疗、APS和OVA混合液治疗组等6组;用OVA致敏,分别用SIT、APS及两者联合等方法治疗,用1%OVA雾化吸入激发;最后一次激发24小时后处死小鼠,进行BALF细胞计数;HE染色观察肺组织切片炎症细胞浸润程度;ELISA法检测血清IFN-γ、IL-4。结果:SIT、APS及两者联合治疗组肺组织炎症细胞浸润程度减轻,BALF中白细胞总数及嗜酸性粒细胞数减少,血清IFN-γ水平上升,IL-4水平下降,联合治疗组比分别治疗组明显。结论:APS可增强SIT的效果,其机制可能是通过影响IFN-γ、IL-4分泌水平实现。
Objective： To explore the effects the of Astragalns Polysaccharide on specific immune therapy in a mouse model of asthma. Methods： Ninety SPF（Specific pathogen Free） BALB/c mice were randomly divided into six groups. The experimental groups were sensitized with OVA, treated with SIT or APS or their joint application. Then the mice were excited with 1% OVA. 24 hours after the last excition, the numbers of the total inflammation cells and esoinophils（EOS） in BALF were counted. The left lung tissues were used to perform histological analysis. IFN-γ and IL-4 in peripheral blood were analysed by ELISA. Results： After the therapy, asthma-associated lung inflammation was inhibited, and the numbers of total cells and EOS decreased. IFN-γ level was raised and IL-4 level was lowered. Conclusion：APS can enhance SIT. It may result from affecting production of both the IFN-γ and IL-4.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2010年第2期132-135,共4页
Chinese Journal of Immunology
基金
广东省中医药管理局课题(No.2050013)
