摘要
目的观察抑制肿瘤细胞骨保护素(OPG)表达对乳腺癌细胞株MDA-MB-231增殖和肿瘤坏死因子相关凋亡诱导配体(tumor necrosis factor-related apoptosis inducing ligand,TRAIL)致其凋亡的影响。方法采用MTT法检测对照MDA-MB-231和MDA-MB-231i细胞(OPG表达抑制)增殖;流式细胞术分析对照MDA-MB-231细胞和TRAIL作用24h后MDA-MB-231、MDA-MB-231i细胞凋亡率。结果前2组细胞倍增时间分别为43.5±2.9小时和45.8±3.6小时,差异无统计学意义;后3组细胞凋亡率分别为6.4%±1.3%、16.1%±1.7%和24.4%±3.3%,每2组之间差异均有统计学意义(P值分别为0.001、0.01和0.018)。结论抑制乳腺癌细胞OPG表达不影响其增殖能力。TRAIL可以诱导乳腺癌细胞凋亡,抑制乳腺癌细胞OPG表达可显著增强TRAIL诱导其凋亡的能力。
Objective To explore the effect of inhibiting osteoprotegerin expression on breast cancer cell line MDA- MB-231 proliferation and TRAIL-induced apoptosis. Methods The proliferations of control MDA-MB-231 cell and MDA- MB-231i cell （osteoprotegerin expression inhibition） were detected by MTT. The apoptosis rate of the control MDA-MB- 231 cell, and the apoptosis rates of MDA-MB-231 cell and MDA-MB-231i cell after 24 hours of TRAIL induction were detected by flow cytometry. Results The doubling generation time in the first two groups was 43.5 ±2.9 hours and 45.8 ± 3.6 hours, respectively （P 〉0.05）. The cell apoptosis rates in the last three groups were 6.4% ±1. 3% , 16. 1% ± 1. 7% , and 24. 4% ±3.3% , respectively. The apoptosis rates between the control MDA-MB-231 cell and the MDA-MB- 231cell, between the control MDA-MB-231 cell and the MDA-MB-231i cell, and between the MDA-MB-231 cell and the MDA-MB-231i cell, were sighificantly different. Conclusion Inhibition of the osteoprotegerin expression does not change the proliferation capability of breast cancer cell. TRAIL can induce breast cancer cell apoptosis, and inhibition of the expression of osteoprotegerin can enhance TRAIL-induced apoptosis in breast cancer cells.
出处
《癌症进展》
2010年第1期95-98,共4页
Oncology Progress
基金
国家自然科学基金资助项目(项目编号:30700814)
第三军医大学2006年度中青年科研基金资助项目
关键词
乳腺癌
骨保护素
增殖
凋亡
breast cancer osteoprotegerin proliferation apoptosis
