摘要
目的研究对比疫苗中蔗糖检验的3种仪器分析方法。方法建立了基于比旋度、高效液相色谱-示差折光检测法和离子色谱-积分脉冲安培检测法对疫苗制品中蔗糖进行质控分析的方法。结果在选定的分析条件下,比旋度、高效液相色谱-示差折光检测法和离子色谱-积分脉冲安培检测法均能准确给出疫苗制品中蔗糖的含量。结论比旋度分析在没有背景干扰的情况下简单易行,高效液相色谱-示差折光检测法和离子色谱-积分脉冲安培检测法则具有更强的专属性及更高的准确度。
OBJECTIVE To study three instrumental analysis methods of sucrose in vaccine product. METHODS The quality control methods of sucrose were performed by specific optical rotation, high performance liquid chromatography-refractive index detection （HPLC-RID） and ion chromatography-integrated pulsed amperometric detection（IC-IPAD）. RESULTS Under the selected conditions, specific optical rotation, HPLC-RID and IC-IPAD methods provided accurately the concentration of sucrose in vaccine product. CONCLUSION Specific optical rotation is easy to implement when matrix interferences don＇t influence the assay. However, HPLC- RID and IC-IPAD methods are more specification and accuracy for the identification and quantitative analysis of sucrose in vaccine product.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2010年第3期218-220,共3页
Chinese Pharmaceutical Journal
关键词
蔗糖
质量控制
旋光
高效液相色谱
离子色谱
疫苗
sucrose
quality control
optical rotation
HPLC
ion chromatography
vaccine
