摘要
目的:探讨乌梅丸对胃癌及癌前病变中端粒酶和PCNA的表达的影响。方法:建立大鼠胃黏膜癌病变模型,采用TRAP-ELISA方法检测大鼠胃黏膜端粒酶活性,检测增殖细胞核抗原(PCNA)的表达。结果:低剂量乌梅丸组(简称低剂量组)中重度异型增生病变数少于模型组,存在统计学差异(P<0.05),高剂量乌梅丸组(简称高剂量组)中重度异型增生病变数少于低剂量组,存在显著性差异(P<0.01);低剂量组端粒酶活性低于模型组,存在统计学差异(P<0.05),高剂量组端粒酶活性低于低剂量组,存在统计学差异(P<0.05);低剂量组PCNA阳性表达率低于模型组,存在统计学差异(P<0.05),高剂量组PCNA阳性表达率低于低剂量组,存在统计学差异(P<0.05)。结论:乌梅丸能抑制端粒酶活性和PCNA的表达,并且呈剂量依赖性,这可能是乌梅丸干预胃癌及癌前病变的机制之一。
Objective： To explore the impact of Wumeiwan on telomerase activity and expression of PCNA in rats＇ gastric cancer and precancerous lesions.Methods： establish rat model of gastric cancer lesions,use the TRAP-ELISA method to detect the telomerase activity in rat gastric mucosa,detect proliferating cell nuclear antigen（PCNA） expression.Results： The low-dose Wumeiwan group（low-dose group） with severe dysplasia in lesions is less than model group,there is a difference in statistics（P 0.05）,high-dose Wumeiwan group（high-dose group） with severe dysplasia in lesions is less than low-dose group,there is a significant difference（P0.01）,telomerase activity in low-dose group lower than model group,there is a significant difference（P0.05）,telomerase activity of high dose group lower than the low-dose group,there is a difference in statistics（P0.05）,the positive expression rate of PCNA in low-dose group is lower than model group,there is a difference in statistics（P0.05）,the positive expression rate of PCNA in high-dose group was lower than the low-dose group,there is statistical differences（P0.05）.Conclusion： Wumeiwan can inhibit telomerase activity and PCNA expression,there is a dose-dependent manner,it may be one of the mechanisms that Wumeiwan interfere with gastric cancer and precancerous lesions.
出处
《中华中医药学刊》
CAS
2010年第2期410-412,共3页
Chinese Archives of Traditional Chinese Medicine
基金
浙江省中医药管理局立项课题(2003C125)
关键词
乌梅丸
胃癌
癌前病变
端粒酶
增殖细胞核抗原
Wumeiwan
gastric cancer
precancerous lesions
telomerase
proliferating cell nuclear antigen
