摘要
目的:采用病例-对照研究,探讨人类白细胞抗原(HLA)-G上游调控区-725单核苷酸多态性与子痫前期的发病是否存在关联。方法:对孕晚期重度子痫前期患者40例、正常足月妊娠妇女41例,提取全血基因组DNA,特异引物巢式聚合酶链反应扩增5’上游调控区,并进行基因测序,对-725位点基因型及等位基因频率进行χ2检验。结果:重度子痫前期组人类白细胞抗原-G基因-725单核苷酸多态性基因型频率GG、GC、CC分别为0%、7.5%、92.5%,正常对照组分别为0%、7.3%、92.7%,两组比较,差异无统计学意义(P>0.05)。重度子痫前期组该位点等位基因频率G、C分别为3.8%、96.2%,正常对照组分别为3.7%、96.3%,两组比较差异无统计学意义(P>0.05)。结论:HLA-G基因-725单核苷酸多态性与中国河南汉族人群重度子痫前期的发病可能没有直接相关。
Objective： To explore the relationship between single nucleotide polymorpbism of human leukocyte antigen - G （ HLA -G） gene upstream regulation region- 725 and pathogenesis of severe preeclampsia by casecontrol study. Methods： Genomic DNA was extracted from peripheral whole blood of 40 cases in late pregnancy with preeclampsia, 41 normal full -term pregnant women, 5＇upstream regulation region of HLA - G gene was amplified by specific primer and polymerase chain reaction, then DNA sequencing was carried out, genotype of - 725 situs and frequency of allele were analyzed by chi - square test. Results ： The frequencies of GG, GC and CC genotypes were 0%, 7. 5% and 92. 5% in severe preeclampsia group, 0%, 7. 3% and 92. 7% in control group, respectively, there was no significant difference （P 〉0.05） . The frequencies of G and C alleles were 3.8% and 96. 2% in severe preeclampsia group, 3.7% and 96. 3% in control group, respectively, there was no significant difference （ P 〉 0. 05 ） . Conclusion ： The single nucleotide polymorphism of HLA - G gene upstream regulation region -725 seems to be disconnected with pathogenesis of severe preeclampsia in Han population in Henan directly.
出处
《中国妇幼保健》
CAS
北大核心
2010年第5期664-666,共3页
Maternal and Child Health Care of China
基金
国家自然科学基金资助项目〔30872316〕
河南省卫生厅重点项目〔200702007〕
