摘要
[目的]探讨乳腺癌组织中浸润树突状细胞及其表面分子表达的意义。[方法]采用免疫组化方法对乳腺癌及周围组织中树突状细胞的表达及其表面分子表达情况进行分析。[结果]26例乳腺癌组织中树突状细胞主要分布于癌周,少量分布于癌灶中;26例患者中有5例肿瘤组织中有CD1 a+、CD83+表达,但均不表达CD54+;癌旁组织中的CD1 a+、CD83+、CD54+表达明显高于乳腺癌组织,差异具有统计学意义(P<0.05)。免疫抑制因子IL-10、TGF-β1的mRNA表达在肿瘤组织中阳性率为76.9%,VEGF A阳性率为65.4%,而癌旁组织中IL-10、TGF-β1的阳性率为26.9%、VEGF A阳性率为11.5%,两者比较差异也具有统计学意义(P<0.05)。[结论]乳腺癌组织中浸润性树突状细胞数量减少及表面分子低表达或不表达可能降低树突状细胞抗原提呈功能,导致机体免疫功能低下。
[ Objective] To investigate the significance of the tumor infiltration of dendritic cells and their surface molecules in the breast cancer. [ Methods ] Twenty - six cases of the breast cancers were studied. The surface molecules of the tumor infiltration of dendritic cells of the cancer tissue were examined by immunohistochemical staining. IL - 10 mRNA,TFG - β1 mRNA, VEGF A mRNA in the tissue and non - tumor tissue were tested by reverse transcript - polymerase chain reaction. [ Results ] The infiltration of dendritic ceils around the cancer mass was found more than that inside the breast cancer tissue. Five cases expressed CD1 a ＋ and CD83 ＋ as positive in the cancer tissue and none expressed CD54＋ as positive in the 26 cases of the breast cancers. In the mRNA level, the positive rates of IL - 10, TGF - β1 and VEGF A in the breast cancer tissue were 76.9% ,76.9% and 65.4% respectively and were higher than them of non - tumor tissue which were down - regulated or had a weak positive expression which were 26.9% ,26.9% and 11.5% （P 〈 0.05）. [ Conclusion] The decrease of the dendritic cell number and the low expression or even non - expression of the surface molecules on the dendritic cells may reduce the antigen - presenting function of the dendritic cells and lead to immune dysfunction against tumor cells.
出处
《大连医科大学学报》
CAS
2010年第1期6-10,共5页
Journal of Dalian Medical University
基金
辽宁省教育厅科学技术研究项目(05L104)
关键词
乳腺癌
树突状细胞
表面分子
breast cancer
dendritic cell
surface molecules
