摘要
利用固相微萃取法收集施用印楝素对褐飞虱诱导的水稻挥发物,并通过气相色谱-质谱联用仪对其进行分离和鉴定.共收集到褐飞虱为害稻株挥发物25种,其中已定性组分14种,以倍半萜类化合物(9种)为主.褐飞虱为害稻株挥发物比健康稻株多柠檬烯、芳樟醇、水杨酸甲酯、未知6、未知7、姜烯、橙花叔醇、正十七烷8种组分.印楝素处理未导致新的挥发物组分产生,但使褐飞虱诱导的挥发物组分相对含量发生变化.印楝素的浓度导致柠檬烯、芳樟醇、水杨酸甲酯、未知6、姜烯、正十六烷6种组分相对含量发生明显变化;而水杨酸甲酯、未知6、未知7、姜烯、橙花叔醇5种挥发物组分相对含量因施药天数不同而存在显著差异;印楝素与水稻品种、害虫密度等生物因子间的互作使所有虫害诱导挥发物组分相对含量均发生显著改变.
With the method of solid phase microextraction （SPME）,a total of twenty-five volatiles were collected from rice plants induced by Nilaparvata lugens,and after applying azadirachtin fourteen of them were qualitatively identified by gas chromatography coupled by mass spectrometry （GC-MS）,mainly of nine kinds of sesquiterpenes.Comparing with healthy rice plants,the plants attacked by N.lugens had more kinds of volatiles,including limonene,linalool,methyl salicylate,unknown 6,unknown 7,zingiberene,nerolidol,and hexadecane.Applying azadirachtin did not result in the production of new kind volatiles,but affected the relative concentrations of the volatiles induced by N.lugens.The proportions of limonene,linalool,methyl salicylate,unknown 6,zingiberene,and hexadecane changed obviously with the concentration of applied azadirachtin,while those of methyl salicylate,unknown 6,unknown 7,zingiberene,and nerolidol changed significantly with the days after azadirachtin application.Azadirachtin concentration,rice variety,and N.lugens density had significant interactions on the relative concentrations of all test N.lugens-induced volatiles.
出处
《应用生态学报》
CAS
CSCD
北大核心
2010年第1期197-202,共6页
Chinese Journal of Applied Ecology
基金
国家自然科学基金项目(30500329)
国家科技支撑计划项目(2006BAD02A03)
苏州市农业科技攻关计划项目(SNG0817)资助
关键词
水稻
印楝素
褐飞虱
虫害诱导的挥发物
rice
azadirachtin
Nilaparvata lugens
herbivore-induced volatile.
