摘要
In this study we studied the factors influencing the callus induction from mature embryos of maize inbred lines Qi 319, Zhen 58, Chang 7 -2, Lx 9801 and 81162, such as genotype, combination of plant growth regulators, and low-temperature pretreatment. The results showed that the induction rate of Qi 319 was the highest among the four genotypes tested; combination of 4.0 mg/L 2,4-D ＋ 0.5 mg/L 6-BA was suitable for inducing callus from mature embryos; three days of 4℃ pretreatment can promote the callus induction significantly. The indices optimized in the present study are helpful for establishing genetic transformation system in maize without considering seasonal variation.
以玉米自交系齐319、郑58、昌7-2、Lx9801和81162的成熟胚为外植体诱导愈伤组织,研究了基因型、外源激素组合、低温处理对愈伤组织诱导的影响。结果表明,基因型对玉米成熟胚愈伤组织诱导的影响显著。在供试的5个基因型中,齐319的愈伤组织诱导率最高,诱导出的愈伤组织质量也较好,为致密、松脆、淡黄色或乳白色,昌7-2不仅愈伤组织诱导率低而且不能产生胚性愈伤组织:在所试验的外源激素组合中,以4,0 mg/L 2,4-D+0.5 mg/L 6-BA对愈伤组织的诱导最为有效;4℃低温预处理3 d对愈伤组织的诱导具有明显的促进作用。经处理,齐319愈伤组织的诱导率由67.5%提高到78.3%。研究确定了愈伤组织诱导的最佳条件,为建立高效的玉米成熟胚再生体系奠定了基础。
基金
Supported by Heilongjiang August First Land Reclamation University(Establishment of fast and high-frequency regeneration system of maize)~~
