摘要
将水貂肠炎病毒基因组分4个重叠片段进行PCR扩增,并将产物克隆到pMD18-T载体中,测定基因组近全长序列。其中编码水貂肠炎病毒非结构蛋白基因(NS1基因)全长为2 007 bp,编码668个氨基酸;编码结构蛋白基因(VP2基因)全长为1 755 bp,编码584个氨基酸。序列分析结果表明:水貂肠炎病毒B株与犬细小病毒、猫泛白细胞减少症病毒NS1序列之间的核苷酸和氨基酸同源性分别为98.8%～99.2%和98.8%～99.2%,与其他细小病毒毒株VP2序列之间的核苷酸和氨基酸同源性分别为98.5%～99.8%和97.8%～99.5%,与MEVantigenic type 2株在亲缘上有着最密切关系。
The complete genome of mink enteritis virus（MEV） was amplified using polymerase chain reaction （PCR） method and cloned. The DNA fragment was sequenced and compared with reference mink enteritis virus. The results showed that the NS1 gene was 2 007 bp length encoding 668 amino acids. The VP2 gene was 1 755 bp length encoding 584 amino acids. The NS1 gene between MEV B strain and other parvovirus strains showed 98.8 %--99.2 % and 98.8 %--99.2 % identities at nucleotide and amino acid levels respectively. The VP2 genes showed 98.5 %--99.8 % and 97.8 %--99.5 % homologies at nucleotide and amino acid levels respectively. The genetic relationship of MEV B strain with MEV antigenic type 2 strain was the closest.
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2010年第1期81-85,共5页
Journal of Jilin Agricultural University
基金
吉林省科技发展计划项目(20075024)
