摘要
目的：建立HPLC法测定芝麻油中芝麻素、芝麻林素含量的方法。方法：以芝麻为原料,通过索式提取,皂化预处理,经石油醚提取、浓缩,甲醇定容,利用高效液相色谱测定不同芝麻原料对应产品中芝麻素、芝麻林素的含量范围。结果：被测两峰完全分离,且峰形较好,线性范围10-100μg/m l,相关系数rsesm in=0.999619 rsesam olin=0.999723。芝麻素、芝麻林素平均回收率分别为102.1%,100.2%。精密度分别为芝麻素的RSD=2.31% 芝麻林素的RSD=3.05%。最低检出限：芝麻素为1.7μg/m l,芝麻林素为2.5μg/m l（以进量浓度计） 对应产品中芝麻素的含量范围为2.5-7.0 mg/g,芝麻林素的含量范围为2.5-4.5 mg/g。结论：该方法简单、准确、灵敏度高、可用于芝麻素、芝麻林素的测定,实验结果可以作为鉴别芝麻油真伪的一种判断依据。
Objective：A new method is established for the determination of sesamin and sesemolin in sesame oil.Methods：Extracted from the sesame by the soxhlet extractor,oil was dealt with saponification,extracted by petroleum ether,concentrated and dissolved in methanol.Thereafter,the sesamin and the sesemolin in the different sesame oil samples were quantitatively determined by high performance liquid chromatography.Results：Good linear relationship was acquired within the range of 10-100 μg/ml（rsesmin=0.999619;rsesamoLin=0.999723）.The recovery of sesamin was 102.1% and sesamolin 100.2%,and the relative standard deviation was 2.31%（sesamin）,3.05%（sesamolin）.The detection limit was 1.7 μg/ml（sesamin）,2.5 μg/ml（sesamolin）in the condition of S/N=3,（according to the concentration of the sample）.The content range of sesamin was 2.5-7.0 mg/g,and the sesamolin was 2.5-4.5 mg/g.Conclusion：The method is rapid,simple,accurate and reproducible.The method can be used for quality assay and the identfication of sesame oil.
出处
《中国卫生检验杂志》
CAS
2010年第1期49-52,60,共5页
Chinese Journal of Health Laboratory Technology
