摘要
本文用膜片箝技术（细胞贴附式和内面向外式）研究了乙醇对大脑皮层锥体神经元膜上延迟整流型钾通道（Ｉｋ）开放概率、开放频率和开放幅度的影响。１５０～５５０ｍｍｏｌ／Ｌ的乙醇抑制Ｉｋ通道开放概率、开放频率，随乙醇浓度增大，抑制作用增强，但不影响通道的电流幅度。４５０ｍｍｏｌ／Ｌ乙醇对Ｉｋ通道开放概率和开放频率的抑制程度在两种记录模式之间差异无显著性。５０ｍｍｏｌ／Ｌ的乙醇不影响Ｉｋ通道的动力学特性。
? Effects of ethanol on delayed rectifier K^+ Channels (48 pS) were studied in acutely dissociated rat cerebral cortical neurons at singlechannel level by cellattached and insideout patch clamp techniques. Ethanol inhibited the Ik channel open probability(nP0) and open frequency in a concentrationdependent manner over the range of 150550 mmol. 50 mmol ethanol has no effect on nPo and open frequency of the channel. No effect was seen on the singlechannel amplitude. The average inhibitions of nP0 and open frequency by 450 mmol ethanol between cellattached and insideout patches are similiar.
出处
《中国应用生理学杂志》
CAS
CSCD
1998年第3期220-223,共4页
Chinese Journal of Applied Physiology
关键词
乙醇
锥体神经元
钾通道
膜片箝技术
ethanol
cerebral cortical neurons
potassium channel
patch clamp
