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JNK信号转导通路在NGF抗6-OHDA诱导PC12细胞凋亡中的作用 被引量：5

JNK signal transduction pathway in anti-apoptotic effect of NGF on PC12 cells treated with 6-hydroxydopamine
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摘要 目的:以6-羟基多巴胺(6-OHDA)作用于大鼠肾上腺嗜铬细胞瘤细胞(PC12cells)以诱导其凋亡,然后在其中分别加入神经生长因子(NGF)及c-Jun氨基端激酶(JNK)阻断剂SP600125,研究在加入NGF后JNK的活性与凋亡的关系。方法:实验分为对照组、6-OHDA组、NGF组、6-OHDA+NGF组、6-OHDA+JNK阻断剂SP600125组,以流式细胞分析法检测各组PC12细胞的凋亡率,以免疫印迹(Westernblotting)法检测各组PC12细胞JNK的活化情况。结果:6-OHDA导致PC12细胞凋亡,JNK1活性提高;预孵SP600125或NGF15min后再加入6-OHDA则PC12细胞凋亡率及JNK1活性均降低。结论:JNK1参与了6-OHDA致PC12细胞凋亡作用,NGF抗6-OHDA所诱导的PC12细胞凋亡作用与其抑制JNK的活化有关。 AIM： To investigate the anti - apoptotic effect of nerve growth factor （NGF） on PC12 cells and to observe the mechanism of signal transduction of JNK pathway. METHODS ： PC12 cells were treated with 6 - hydroxydopamine （6 -OHDA） to induce cell apoptosis. NGF and SP600125, the c -Jun N -terminal kinase inhibitors, were added respectively in order to study the relationship between the activity of c - Jun N - terminal kinase and apoptosis of PC12 cells. The cells were divided into control group, 6 - OHDA group, NGF group, 6 - OHDA plus NGF group, NGF plus SP600125 group. The apoptotic rates of PC12 cells with different treatments were detected by flow cytometry and activities of JNK in PC12 cells were determined by Western blotting. RESULTS： 6 - OHDA increased the apoptotic rate and activity of JNK1 in PC12 cells. Incubation with SP600125 or NGF for 15 min before adding 6 - OHDA decreased the apoptotic rate of PC12 cells and activity of JNK1 in PC12 cells. CONCLUSION： Activity of JNK is involved in the pro -apoptotic effect of 6 - OHDA on PC12 cells. Anti - apoptotic effect of NGF on PC12 cells affected by 6 - OHDA is related to the decrease in the activity of JNK1.
作者 汪军兵 胡景鑫
机构地区 荆楚理工学院医学院病理教研室 广州医学院生理教研室
出处 《中国病理生理杂志》 CAS CSCD 北大核心 2010年第2期277-281,共5页 Chinese Journal of Pathophysiology
关键词 大鼠 PC12细胞 细胞凋亡 6-羟基多巴胺 C-JUN氨基末端激酶 神经生长因子 Rats PC12 cells Apoptosis 6 - hydroxydopamine c - Jun N - terminal kinase Nerve growth factor
分类号 R338 [医药卫生—人体生理学]
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参考文献17

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共引文献27

同被引文献51

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中国病理生理杂志
2010年 第2期

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