摘要
目的：探讨多脏器功能失常综合征发病中细胞凋亡的作用。方法：建立大鼠肠系膜上动脉夹闭模型，常规组织切片，ＨＥ染色，普通光学显微镜检查及吖啶橙溴化乙锭双重染色的荧光显微镜观察肠、肝、肺、肾４个器官组织的细胞死亡现象，并在石蜡切片上计数凋亡细胞的百分比；荧光显微镜下计数缺血再灌注不同时间分离的肝细胞中活细胞、凋亡细胞和坏死细胞数。结果：①肠缺血再灌注后各时间点均可观察到细胞凋亡及细胞坏死现象；②局灶性的肾小球和肾小管坏死主要分布在肾皮质与髓质交界处；③肝细胞凋亡和细胞坏死的百分比均随肠缺血再灌注时间延长而升高，且与假手术组比较均有显著性差异（Ｐ均＜０．０５）；缺血１２０分钟、再灌注６０分钟组与缺血１８０分钟组比较，细胞凋亡与细胞坏死的百分比均较高（Ｐ均＜０．０５）。结论：肠缺血再灌注后多个器官组织在短时间内即可发生大量细胞凋亡。
Objective:To elucidate the potential role of apoptosis in the pathogenesis of multiple organ dysfunction syndrome.Methods:Using a superior mesenteric artery occlusion rat model,cell death in intestine,lung,liver,and kidney was observed by light microscopy in HE stained tissue sections and by fluorescence microscopy in tissue sections stained with acridine orange and ethidium bromide.The percentage of apoptotic cells in normal tissue sections was counted under light microscope,and the percentage of apoptotic and necrotic cells in isolated hepatocytes were counted under fluorescence microscope at different time points.Results:Both apoptotic and necrotic cell death were able to be detected after intestinal ischemiareperfusion.Focal necrotic and apoptotic glomeruli and renal tubules distributed mainly at the cortexmedulla junction.Percentages of hepatocyte apoptosis,necrosis and the rate of apoptosis were gradually increased along with prolongation of intestinal ischemiareperfusion,which were significantly different from shamoperated groups (all P <0 05).Also,percentage of cell apoptosis and necrosis in 2 hoursischemia followed by 1 hourreperfusion group was higher than that of 3 hoursischemia group (all P <0 05).Conclusions:Cell apoptosis and necrosis in multiple organs would occur shortly after intestinal ischemiareperfusion,which might play an important role in the pathogenesis of multiple organ dysfunction syndrome.
出处
《中国危重病急救医学》
CAS
CSCD
1998年第10期623-626,共4页
Chinese Critical Care Medicine
基金
全军"九五"医药卫生指令性研究课题
关键词
肠缺血
再灌注损伤
细胞凋亡
细胞坏死
MODS
intestinal ischemiareperfusion injury\ \ apoptosis\ \ necrosis\ \ multiple organ dysfunction syndrome
