摘要
目的：研究测定微量钴的新体系。方法：分光光度法。结果：在ｐＨ９３～９８介质中，在表面活性剂ＣＴＭＡＢ和ＴｒｉｔｏｎＸ－１００存在下，Ｃｏ（Ⅱ）与４，５－二溴邻硝基苯基荧光酮（ＤＢ－ｏ－ＮＰＦ）形成组成比为１∶３蓝紫色配合物，其最大吸收波长６２０ｎｍ，表观摩尔吸光系数ε＝１４１×１０５，Ｃｏ（Ⅱ）含量在０～５μｇ／２５ｍｌ范围内符合比尔定律。结论：建立了测钴的分光光度新方法。该法操作简便，灵敏度高，可直接用于ＶＢ１２中钴的测定。
Objective:To study a new system of determining trace Cobalt(Ⅱ).Method:Spectrophotometry.Results:In a medium of pH 93～9.8,Cobalt(Ⅱ) reacts with 4,5-dibromo-o-nitrophenyl fluorone (DB-o-NPF) to form a 1:3 complex with maximum absorption at 620nm in the presence of CTMAB and Triton X-100.The molar absorptivity is 1.41×105.Beers law is obeyed in the range of 0～5μg 25ml.Conclusion:A new method for determining trace Cobalt(Ⅱ) was established.The method has been used to determine trace Cobalt(Ⅱ) directly in VB12 with satisfactory results.
出处
《内蒙古医学院学报》
1998年第3期175-178,共4页
Acta Academiae Medicinae Neimongol
