摘要
目的探讨乙型肝炎后肝硬变（ＰＨＣ）患者外周血淋巴细胞（ＰＢＬ）ＩＬ２活性，ＩＬ２Ｒ表达与肝功能的关系．方法ＰＨＣ患者５６例，按ＣｈｉｌｄＰｕｇｈ分级法将其肝功能状态分成Ａ，Ｂ，Ｃ三级，检测其ＩＬ２Ｒ的表达，ＩＬ２的生成及血清可溶性ＩＬ２受体（ｓＩＬ２Ｒ）的水平，并与２４例健康成人进行比较．结果ＰＨＣ组ＰＢＬ经ＰＨＡＰ诱导的ＩＬ２Ｒ表达和ＩＬ２生成均显著低于对照组（ｔ＝２２７０和４６３３，Ｐ＜００５和Ｐ＜００１），而血清ｓＩＬ２Ｒ水平则显著高于对照组（ｔ＝６２１９，Ｐ＜００１）．ＰＨＣ组和对照组的ＩＬ２活性与ＩＬ２Ｒ表达率之间呈正相关（ＰＨＣ组：ｒ＝０４１４，Ｐ＜００１；对照组：ｒ＝０８０４，Ｐ＜００１），与血清ｓＩＬ２Ｒ水平则呈负相关（ＰＨＣ组：ｒ＝－０５０６，Ｐ＜００１；对照组：ｒ＝－０４２２，Ｐ＜００５）．ＰＨＣ患者随着肝功能恶化（ＣｈｉｌｄＰｕｇｈＡ，Ｂ，Ｃ三级之降低），ＰＢＬ经ＰＨＡＰ诱导的ＩＬ２Ｒ表达和ＩＬ２生成亦越低，而ｓＩＬ２Ｒ水平则越高．结论ＰＨＣ患者ＩＬ２Ｒ系统的功能有障碍与缺陷，而且这种障碍与缺陷与其肝功能状态有密切关系?
AIM To explore the correlation between liver function states and IL 2 activity and IL 2R expression in patients with post hepatitis B cirrhosis (PHC). METHODS IL 2 production and IL 2R expression on peripheral blood lymphocytes (PBL) induced by PHA P, the serum level of soluble IL 2 receptor in 56 patients with PHC and in 24 healthy adults as control were measured by the modified MTT method, flow cytometry assay and ELISA method, respectively. RESULTS〓IL 2R expression and IL 2 production in PHC patients were much lower than those in the control group ( t =2 270 and 4 633, P <0 05 and P <0 01 , respectively), but serum sIL 2R level was significantly higher than that in the control group ( t =6 219, P <0 01 ). A significant linear correlation was found between IL 2 production and IL 2R expression (PHC group: r =0 414 , P <0 01 ; control group: r =0 804 , P <0 01 ), while a significant reverse correlation was found between IL 2 activity and serum sIL 2R level (PHC group: r =-0 506 , P <0 01 ; control group: r =-0 422 , P <0 05 ). With Child Pugh's classification of cirrhosis, we found that the poorer the liver function, the lower the IL 2R expression and IL 2 production, and the higher the sIL 2R level. CONCLUSION The function of IL 2 system in patients with PHC was decreased and closely correlated with their liver function state.
关键词
乙型肝炎
肝硬变
IL-2
IL-2R
肝功能
hypertension, portal/physiopathology
stomach/physiopathology
gastric mucosa/physiopathology
