摘要
目的探讨单核细胞(MO)呼吸爆发过程中产生的反应性氮代谢物(RNM)、反应性氧代谢物(ROM)的情况及其对NK细胞抗K562细胞活性的影响。方法在NK+K562混合细胞培养体系中,观察加入MO或/和IL-2/PHA前后RNM、ROM产量、KIR及TNF-β、IFN-γ产量的相应变化。再观察加入二氢氯组胺、硫普罗宁后上述各指标的变化情况。结果①在NK+K562混合培养体系中,加入IL-2/PHA后,KIR逐渐升高;当按E/MO=10/2、10/5、10/10比例加入MO后,RNM、ROM产量随着MO数量的增加而增加,而TNF-β、IFN-γ的产量和KIR反而降低。对K562细胞数量作偏相关分析,RNM与KIR的负相关系数大于ROM。②加入药物后,ROM产量明显下降,KIR和TNF-β、IFN-γ产量明显升高,硫普罗宁还降低RNM的产量,但二氢氯组胺无效。结论①MO可通过产生ROM、RNM降低NK细胞的活性,抑制NK细胞抗K562细胞效应;②RNM对NK细胞的影响可能比ROM更强。③硫普罗宁通过清除ROM、RNM,可逆转MO对NK细胞的抑制作用。
To explore the diversify of reactive nitrogen metabolites（RNM）/reactive oxygen metabolites（ROM） and their influence on anti-K562 cell activity of NK cell during the monocytes respiratory burst.In the co-cultivation system of NK cells and K562 cells,monocytes or/and IL-2/PHA were administered,then the corresponding changes of RNM/ROM and TNF-β/IFN-Υproduction,as well as K562 cell inhibition rate（KIR） were observed.Besides,histamine dihydrochloride（DHT） and tiopronin（TIP） were administered in the culture system and the indexes above were observed again.After IL-2/PHA was administered in the cultivated systems mixing with NK cells and K562 cells as the E/T（NK/K562） ratio was 10/1,KIR increased gradually;after monocytes being appended as E/MO（NK/monocyte） ratio was 10/2,10/5,and 10/10,RNM and ROM productions increased correspondingly,but TNF-β/IFN-Υproduction and KIR decreased. Controlling by K562 cells,the negative correlation coefficient of RNM to KIR exceeds that of ROM to KIR.After administering DHT and TIP,expecially TIP,the ROM production decreased but the TNF-β/IFN-Υproduction and KIR increased.Morover,TIP could decrease the RNM production but DHT failed.All the results suggest that monocytes could product a lot of ROM and RNM to degreded NK cells activity and disable NK cells in killing K562 cells,in which RNM may play more important role than ROM;TIP can reverse the depressant effect of MO on NK cell by scavenging ROM and RNM.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2010年第1期29-33,共5页
Immunological Journal
基金
福建省科技厅资助省属高校项目(2007F5043)
