摘要
目的 探讨血小板衍生生长因子 (PDGF)在表皮角朊细胞和真皮成纤维细胞的产生及其调节因素。方法 培养人角朊细胞和成纤维细胞 ,用ELISA法测定PDGF多肽分子 ,用Northern杂交检测PDGF B链mRNA的表达。结果 人角朊细胞可合成PDGF多肽分子 ,双羟维生素D3 可促进其合成 ,肉豆寇沸波酯 (PMA)可抑制其合成。成纤维细胞不产生PDGF分子 ,可测到低水平的PDGF B链mRNA表达。结论 表皮角朊细胞是皮肤中PDGF分子的一个重要产生细胞 。
Objective To evaluate the production and regulation of platelet derived growth factor (PDGF) in the skin. Methods Normal human keratinocytes (NHKs) and human dermal fibroblasts (HDFs) were cultured, PDGF peptides were measured by ELISA and PDGF B chain mRNA was detected by Northern hybridization. Results The results showed that NHKs constitutively produced PDGF molecules, phorbol 12 myristate 13 acetate (PMA) inhibited PDGF production, while 1, 25 dihydroxyvitamin D 3 enhanced its production. TNF alpha, interferon γ and interleukin 1 alpha all failed to affect PDGF production. On the other hand, HDFs produced no PDGF molecule in spite of a low level of PDGF B chain mRNA expression in Northern hybridization, suggestting a post transcriptional blocking of PDGF production in HDFs. Conclusion The results obtained above indicate that epidermal keratinocytes may be the major PDGF generating cells, whereas dermal fibroblasts the PDGF responsive cells in the skin.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
1998年第4期233-235,共3页
Chinese Journal of Dermatology
