摘要
对2006—2007年分离于江苏省部分发病猪场的PRRSV,采用RT-PCR技术,进行ORF5基因序列以及Nsp2基因部分序列的扩增、克隆并测序。应用DNAStar软件将测序结果与已发表的国内外参考毒株进行比对分析。结果表明,分离的21株病毒ORF5基因大小均为603bp;与国内分离株之间同源性为87.7%～97.0%,与国外美洲型分离株同源性为85.6%～90.5%;21株PRRSV分离株与欧洲型毒株之间的同源性仅为54.1%～56.6%。14株用于扩增Nsp2基因部分序列的PRRSV在全基因组第2778～2780位及第2947～3033位分别缺3个和87个碱基,其中的2个江苏扬州分离株在第2747～2836位又缺失了90个碱基;14个毒株之间同源性为93.2%～99.0%,与VR-2332相比同源性为74.9%～78.5%,与国内河北、河南等地2006年的PRRSV分离株相比同源性为94.1%～99.1%。
ORF5 of 21 PRRSV isolates from Jiangsu province of China in 2006-2007 and Nsp2 gene of 14 isolates were amplified by RT-PCR. The PCR products were cloned and sequenced. The results showed that ORF5 gene of all the isolates were 603 bp in length. The 21 isolates had an identity of 98.5%-100.0% ,simultaneously had an identity of 87.7%-97.0% and 85.6 %-90.5 % with domestic isolations and American strains, respectively, the 21 isolates has an identity of 54.1%-56.6% with European strains. According to Nsp2 genome analysis,deletions of 87 bases at 2 947-3 033 and 3 bases at 2 778 2 780 of the full-length genome of VR-2332 were found in all the 14 isolates. Besides, 90 bases at 2 747-2 836 were deleted in two of the fourteen isolates from Yangzhou. There was an identity of 93.2%- 99.0% within the 14 isolates,they showed an identity of 74.9%-78.5%0 and 94. 1%-99.1% with VR-2332 and isolates from Hebei and Henan of China,respectively.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2010年第2期145-149,共5页
Chinese Journal of Veterinary Science
基金
国家"十一五"科技支撑计划资助项目(2006BAD06A12)
