摘要
从高表达GST/MCP-1的菌株纯化GST/MCP-1,其表达产物以包涵体形式存在.包涵体经分离洗涤后,探索了对其变性复性的最佳条件.复性后的样品经Glutathione Sepharose 4B一步亲合层析纯化,获得了具有生物学活性的SDS-PAGE纯的GST/MCP-1.Western Blot检测表明,纯化的GST/MCP-1可与MCP-1抗体发生特异反应.体外抗肿瘤试验表明,CST/MCP-1激活单核细胞及淋巴细胞后,具有抑制肿瘤细胞生长的能力.裸鼠抗肿瘤试验同样显示明显的抑制反应.上述结果提示MCP-1具有抗肿瘤能力.
GST/MCP-1 fusion protein was overexpressed in Escherichia coli as inculstion bodies. After isolation of the inclusion bodies, the optimum conditions of denaturation and renaturation were studied. The renatured produts were purified to be electroporetically pure by affinity chromatography on immobilised glutathione. The purified product remains biological activities and can react specifically with MCP-1 antibodies by western blot analysis. The in vitro antitumor effect showed that GST/MCP-1 could activate monocytes and lymphocytes to inhibit the growth of a lung adenocarcinoma cell line A549. In vivo, GST/MCP-1 could inhibit the tumor growth in nude mice. These results suggested that MCP-1 had antitumor effect.
出处
《中国肿瘤生物治疗杂志》
CAS
CSCD
1998年第3期208-211,共4页
Chinese Journal of Cancer Biotherapy
