摘要
目的 :探讨人参水提液 (简称人参液 )对大鼠慢性绿脓杆菌 (PA)性肺炎模型的影响。方法 :采用 4种不同剂量的人参液皮下注射 ,观察用药两周后的疗效。结果 :4种剂量的人参液均可显著减少肺部大体观病变和肺脓肿率 (P <0 0 5) ;镜下观其病变程度与人参剂量呈负相关 (r=-0 95,P <0 0 1 ) ;人参Ⅰ (5mg/kg)、Ⅱ (2 5mg/kg)、Ⅳ (0 6 2 5mg/kg)组的肥大细胞数低于对照组 (P <0 0 5) ;Ⅱ、Ⅲ (1 2 5mg/kg)组的肺部细菌清除作用强于对照组 (P <0 0 5) ;Ⅱ、Ⅲ、Ⅳ组的PA特异性抗体应答发生下调 (P <0 0 1 ) ;Ⅰ组的IgM水平低于对照组(P <0 0 1 ) ,且IgM水平的高低与人参剂量呈正相关 (r =0 93,P <0 0 5) ;Ⅰ组的IgG2a水平高于对照组 (P <0 0 5) ;血清IgG2a水平与人参剂量呈正相关 (r =0 90 ,P <0 0 2 ) ;PA感染两周后 ,Ⅰ、Ⅱ、Ⅲ组的抗藻酸盐IgG水平高于对照组和Ⅳ组 (P <0 0 5) ,而Ⅰ、Ⅲ、Ⅳ组的IgA则低于对照组 (P <0 0 5)。结论 :2 5mg/kg的人参是本研究的最佳剂量 。
Objective: To observe the effect of ginseng extracts on a rat model of chronic Pseudomonas aeruginosa (PA) pneumonia. Methods: Subcutaneous injection with four different doses of ginseng aqueous extracts to the model rats for two weeks. Results: Milder macroscopic lung pathology and lower incidence of lung abscesses were found in all the four groups received different doses of ginseng treatment compared to the control group (P<0 05). Histopathology of the lungs was negatively correlated with the ginseng doses (r=-0 95,P<0 01). The mast cell number in the lung foci in group Ⅰ, Ⅱ and Ⅳ was significantly lower than that in the control group (P<0 05). Bacterial clearance in group Ⅱ and group Ⅲ was enhanced significantly (P<0 05) compared with that in the control group. The specific antibody responses were down regulated in group Ⅱ, Ⅲ and Ⅳ (P<0 01), and IgM level in group Ⅰ was lower compared to the control group (P<0 01). Serum IgM level was positively correlated with the ginseng doses (r=0 93,P<0 05). IgG2a titer in group Ⅰ was higher than that in the control group (P<0 05). Serum IgG2a level was positively correlated with the ginseng doses (r=0 90,P<0 02). Two weeks after PA lung infection, the serum level of IgG against PA, aeruginosa alginate in group Ⅰ, Ⅱ, and Ⅲ was higher than that in the control and group Ⅳ (P<0 05), and the anti alginate IgA level in group Ⅰ, Ⅲ and Ⅳ was lower than that in the control (P<0 05). Conclusions: The ginseng dose of 2.5 mg/kg was the best dosage in the present study, and it showed an effect of stimulating T helper type Ⅰ response.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
1998年第9期546-549,共4页
Chinese Journal of Integrated Traditional and Western Medicine
关键词
人参水提液
绿脓杆菌
肺炎
剂量
中医药疗法
ginseng aqueous extract
Pseudomonas aeruginosa
lung infection
cystic fibrosis
