摘要
目的：对HLA－DQα和PM位点进行多态性研究并应用于实际检案。方法：用PCR结合反相杂交技术进行扩增并分型。结果：获得HLA－DQα和PM位点的基因频率分布数据。结论：上述位点具有高度多态性，累积个体鉴别能力（DP值）达99．95％，是法医学亲权鉴定和个体识别领域非常有价值的遗传标记系统。
Studies were conducted to evaluate the forensic ap-plicability of multiplex amplification of HLA -DQα and PM loci. These loci could be simultaaneously typed by re-verse dot blot approach where allele specificoligonucleoti-de probes were immobilized on a nylon membrane strip.Allele and genotype frequencies for HLA -DQα and PMloci were determined in Chinese Han population . The fre- quency data can be used in individual identification andp paternity test.
出处
《法医学杂志》
CAS
CSCD
1998年第3期138-140,共3页
Journal of Forensic Medicine
关键词
PCR
复合扩增
HLA-DQα
PM
法医物证学
：PCR Multiplex Amplification，Reverse Dot Blot, Gene Loci， HLA -DQα, LDLR, GYPA, HBGG,D7S8, GC
