摘要
目的观察颅内灌注Aβ1-42和Thiorphan给药后恒河猴基底、核大脑皮质等部位胆碱能神经元的改变。方法将恒河猴4只,分为对照组(1只)与实验组(3只),对照组开颅后只注射0.9%氯化钠溶液,实验组开颅后先注射Thiorphan到猕猴的基底核和大脑皮质消耗已存在的Neprilysin,然后再缓慢的注射孵育好的纤丝状Aβ1-42,再植入含有Thiorphan的微渗透泵到基底核。免疫组织化学方法检测大脑切片胆碱乙酰转移酶(ChAT)免疫反应活性及阳性神经元数量及形态学改变。结果给药后实验组恒河猴基底核、皮质等部位的胆碱能神经元数量减少和ChAT水平降低。结论 Aβ1-42和Thiorphan联合颅内给药后损害大脑基底核、皮质等部位的胆碱能神经元。
Objective To observe the effects on cholinergie neurons in Macaca Rhesus model induced by intraeranial co-infusion of Aβ1-42 and Thiorphan. Methods After drilling the skull, the Thiorphan was injected into the cerebral cortex and into the basal ganglia to consume the existed Neprilysin, then infusing the fibrilla Aβ1-42 slowly and implanting miniosmotic pump with Thiorphan into the basal ganglia. The pathological changes and number of immunoreactive neurones were observed, and the activity of ChAT was detected by immunohistochemistry. Results The number and levels of ChAT positive neuros in basal ganglia and cerebral cortex of Macaca Rhesus brain decreased significantly in experimental group, as compared with those in controll group. Conclusion The combined application of Aβ1-42 and Thiorphan in Macaca Rhesus brain can damage the eholinergic neurons in basal ganglia and cerebral cortex of Maeaea Rhesus brain.
出处
《河北医药》
CAS
2009年第23期3181-3184,共4页
Hebei Medical Journal
基金
广东省自然科学基金重点攻关项目(编号:010086)
广东省科技计划项目(编号:2006B36004015)
广东省自然科学基金自由申请项目(编号:06023013)
关键词
恒河猴
阿尔茨海默病
胆碱乙酰转移酶
免疫组化
Macaca Rhesus
Alzheimer disease
choline acetyhransferase
immunohistochemistry
