摘要
［目的］初步探讨红酵母原生质体制备和再生的条件。［方法］以红酵母为试材，研究渗透压稳定剂pH值、酶浓度、酶解时间、预处理时间4个因素对红酵母原生质体制备率和再生率的影响。［结果］pH值在6.5～7.0，原生质体的制备率和再生率随着渗透压稳定剂pH值的升高而上升。当酶浓度从0.2%上升到1.0%时，原生质体制备率上升较快，而原生质体再生率下降较慢。随着酶解时间的增加，原生质体的制备率逐渐增加，而原生质体的再生率逐渐下降。在5～15 min，原生质体制备率和再生率均随着预处理的时间的增加而降低。［结论］当渗透压稳定剂pH值为7.0，蜗牛酶酶浓度为1.0%，酶解时间为60 min，预处理时间为5 min时，原生质体的制备率和再生率能分别达到较高的值。
[ Objective ] The aim was to preliminarily research the protoplast preparation and regeneration condition of rhodotorula. [ Method] With the rhodotorula as the tested material, the effects of four factors including the pH value of osmotic stabilizer, enzyme conch. , enzymolysis time and pretreatment time on the preparation and regeneration rate of Rhodotorula protoplast were studied. [ Result ] The protoplast prepara- tion rate and regeneration rate were raised with the rising of the pH value of osmotic stabilizer when the pH value was in the range of 6.5 - 7.0. The protoplast preparation rate was raised faster but the protoplast regeneration rate was descended slower when the enzyme eonen, was raised from 0.2% to 1.0%. With the rising of the enzymolysis time, the protoplast preparation rate was increased gradually, but the protoplast regeneration rate was decreased gradually. The protoplast preparation rate and regeneration rate all were decreased with the rising of pretreatment time in the range of 5 -15min. [ Conclusion ] The protoplast preparation rate and regeneration rate were up to the higher value resp. when the pH value of osmotic stabilizer, the enzyme concn, of snail enzyme, enzymolysis time and pretreatment time was 7.0, 1.0%, 60 min, 5 rain resp.
出处
《安徽农业科学》
CAS
北大核心
2010年第3期1154-1155,1172,共3页
Journal of Anhui Agricultural Sciences
基金
玉林师范学院院级重点资助项目(2007YJZD09
2008YJZD-14)
广西教育厅资助项目(200709LX145)
关键词
红酵母
原生质体制备
原生质体再生
Rhodotorula
Protoplast preparation
Protoplast regeneration
