摘要
目的:建立以液-质联用法测定人血浆中匹卡米隆浓度的方法。方法:色谱柱为Agilent C18,流动相为甲醇-水(85∶15),流速为0.2mL·min-1,柱温为20℃,进样量为10μL,内标为瑞格列奈;用于定量分析的离子反应分别为m/z207.0→m/z121.1(匹卡米隆)和m/z451.3→m/z379.2(瑞格列奈)。结果:匹卡米隆血药浓度在50～10000μg·L-1范围内线性关系良好(r=0.9991);方法回收率在99.14%～104.27%之间,日内和日间RSD分别为2.12%～5.94%、7.30%～8.44%。结论:本方法灵敏、准确,可用于匹卡米隆的血药浓度监测及其药动学研究。
OBJECTIVE： To establish an LC-MS method for determination of picamilon in human plasma. METHODS： Picamilon and repaglinide（as internal standard）were separated on Agilent C18 column, using methanol-water（85 ： 15） as the mobile phase at a flow rate of 0.2 mL. rain 1. Column temperature was set at 20 ℃ SIM was used for determination of picamilon in human plasma.Ion mass spectral （m/z） of 207.0→121,1 was selected for picamilon and 451.3→379.2 for repaglinide .RESULTS： The linear range was 50-10000 μg· L^-1（r=0.999 1） ; the method recovery was within 99.14%-104.27%. The RSD of inter-day and intra-day validation were 2.12%-5.94% and 7.30% -8.44%, respectively. CONCLUSION： The method is sensitive and accurate for the determination of picamilon in human plasma and its pharmacokinetic study.
出处
《中国药房》
CAS
CSCD
北大核心
2010年第2期133-135,共3页
China Pharmacy
