摘要
采用FISH技术对2008年冬、夏两季太湖梅梁湾水体中细菌优势种群的丰度进行了对比分析。实验中,选取荧光素标记的特异寡核苷酸探针,与太湖梅梁湾水体中的四类细菌优势种群α-变形菌(α-proteobacteria),β-变形菌(β-proteobacteria),γ-变形菌(γ-proteobacteria)和拟杆菌(Bacteroidetes)进行原位杂交。结果表明:①所测定的四类细菌优势种群占总细菌的比例在冬季均显著高于夏季(P<0.01);②水体中α-proteobacteria的丰度随TP浓度的增加而显著增加(R^2=0.860,P<0.01);③随着Chl.a浓度的增加,水体中细菌的丰度呈上升的趋势,尤其是其中的γ-proteobacteria,其增大趋势更加显著(R^2=0.857,P<0.01);④细菌种群的丰度受水体中PO_4^(3-)浓度的影响,尤其是对Bacteroidetes的影响更加显著(R^2=0.733,P<0.05)。
FISH was used to analyze the abundance of dominated bacteria population in Meiliang Bay of Lake Taihu in winter and summer in 2008. The four dominated bacteria population （ α-proteobacteria, β-proteobacteria, T-proteobacteria, Bacteroidetes） were analyzed by in situ hybridization with specific fluorescently （FITC） labeled rRNA-targeted oligonucleotide probes in the test. The results indicated ： （1） The proportion of each of the four dominated species in the total number of bacteria was all significantly higher in winter than that in summer（ P 〈0. 01 ） ; （2） the abundance of α-proteobacteria rose notably with the increase of TP concentration （ R^2 = 0. 860, P 〈 0. 01 ） ; （3） there was ascending trend in the abundance of bacteria along with the increase of Chl. a concentration, especially that of γ-proteobacteria was more remarkable than the others （ R2 = 0. 857, P 〈 0. 01 ） ; （4） the abundance of bacteria population, especially for Bacteroidetes, was also influenced by PO4^3 - concentration （R^2 = 0. 733, P 〈 0. 05 ）.
出处
《湖泊科学》
EI
CAS
CSCD
北大核心
2010年第1期57-62,共6页
Journal of Lake Sciences
基金
中国科学院知识创新工程重要方向性项目(KZCX2-YW-419)
国家重点基础研究发展计划项目(2008CB418103)
国家自然科学基金项目(40730529)联合资助
