摘要
目的了解湘潭地区乙型肝炎病毒(HBV)基因型流行情况及其临床意义,并对HBV基因分型试剂盒进行评价。方法用HBV基因分型试剂对湘潭地区95例HBV感染者的样本进行检测,并对PCR产物进行测序分析。结果 HBV基因分型试剂盒检测结果与测序的符合率为100%,湘潭地区HBV各种基因型分布比例:B型占74.74%,C型占11.58%,B、C型混合感染占13.68%。无症状携带者组与慢性重型肝炎组.慢性乙型肝炎组与慢性重型肝炎组及肝硬化组之间HBV B型和C型感染率存在显著性差异,其中慢性重型肝炎组及肝硬化组C型感染率要明显高于其他组。结论 HBV基因分型检测试剂盒检测结果是准确、简便的,具有临床推广和应用价值。湘潭地区流行的HBV基因型主要为B型和C型,其中B型为优势基因型,具有其自身的特点。
Objective This study was performed to investigate the distribution of hepatitis B virus genotypes in Xiangtan and its clinical significance. Through the study HBV genotyping kit was evaluated. Methods PCR- reverse dot-blot by HBV genotyping kit and DNA sequencing were carried out to determine the HBV genotype of the specimens from 95 patients which were infected by HBV. Results The results of obtained from PCR-reverse dot-blot by HBV genotyping kit were consistent with those of sequence analysis. The prevalence of genotype B was 74.7% （71/95）, genotype C was 11.6% （11/95） and mixed of genotype B and C was 13.6% （13/95）. Conclusion The results of PCR-reverse dot-blot by HBV genotyping kit are accurate, while its operating procedures are easy. This kit can be promoted and used in the clinical laboratory. The predominant HBV genotypes in Xiangtan were genotype B, C and B＋C, in which the major genotype in Xiangtan was genotype B.
出处
《热带医学杂志》
CAS
2009年第12期1385-1387,共3页
Journal of Tropical Medicine
