摘要
目的：细胞遗传学研究发现，鼻咽癌染色体７ｑ３２－ｑｔｅｒ常有缺失。为进一步证实鼻咽癌在这个染色体区域的遗传变化。方法：选择位于７ｑ３１３～３６的１３个微卫星标记对２４例鼻咽癌进行等位基因缺失分析。结果：７９％（１９／２４）的鼻咽癌至少在一个位点存在等位基因丢失，高频缺失位点是Ｄ７Ｓ４９５（４６％），Ｄ７Ｓ５００（４５％），Ｄ７Ｓ６３１（３０％）和Ｄ７Ｓ５１４（３５％）。依据缺失图谱分析，一个较小的共同缺失区域可能存在于７ｑ３２的Ｄ７Ｓ４９５和Ｄ７Ｓ５００之间，其遗传距离约为９６ｃＭ。且这个小区域的缺失与鼻咽癌的临床分期无显著相关性（Ｐ＞００５）。结论：这些结果提示，鼻咽癌在７ｑ３２的Ｄ７Ｓ４９５与Ｄ７Ｓ５００之间可能存在与鼻咽癌相关的抑瘤基因。
Objective:To better define genetic alterations of chromosome 7q32-qter,because the deletions of chromosome 7q32-qter are commonly observed in nasopharyngeal carcinoma (NPC). Methods: PCR-assays for the analysis of 13 microsatellite markers mapped to chromosome 7q31 3～36 were performed on 24 NPC patients. Results:Allelic imbalance and loss of hetreozygosity were found in 19 of the 24 tumor specimens (79%) at least one locus.Higher frequent allelic losses were identified at four loci,which were D7S495(46%),D7S500(45%),D7S631(30%) and D7S514(35%).A smaller commonly deleted region was supposed to be present between D7S495 and D7S631 at 7q32 and their genetic distances might be within 9.6 centimorgan.The LOH/deletion on chromosome 7q32 did not correlate with the clinical staging of NPC( P >0 05).Conclusion:A putative tumor suppressor gene may be present at a smaller region within 10cM on chromosome 7q32, which may be important for cancer initiation and early development in nasopharyngeal carcinoma.
出处
《中国耳鼻咽喉颅底外科杂志》
CAS
1998年第3期165-171,共7页
Chinese Journal of Otorhinolaryngology-skull Base Surgery
基金
国家863高技术项目
关键词
鼻咽肿瘤
微卫星多态性
7号染色体
杂合性丢失
Allelic loss Microsatellite polymorphism Chromosome7q Nasopharyngeal neoplasm
