摘要
利用固相酶染色法(SESA)和试管玻片凝集法(F-D 法)分别检测不孕母牛(试验组)和处女牛、正常空怀及妊娠母牛(对照组)血清和子宫颈粘液的抗精子抗体(AsAb)反应。结果表明,SESA 测定不孕母牛血清 AsAb 平均阳性反应率显著高于处女牛和空怀及妊娠母牛(P<0.001)。不孕母牛子宫颈粘液的 AsAb 阳性反应率亦高于对照组母牛(P<0.05)。不孕母牛 AsAb 阳性反应血清和子宫颈粘液的滴度分别为1∶133和1∶141,两者差异不显著(P>0.05)。F-D 法检测的结果,试验组血清 AsAb 阳性反应率高于处女牛(P<0.05),但与空怀及妊娠母牛比较无统计差异(P>0.05)。两种检测方法均未发现不孕母牛配种次数和胎次与AsAb 阳性反应率之间的明显相关。试验表明:母牛血清和生殖道的 AsAb 存在与繁殖状态有关,可能是母牛某些不孕症的免疫学原因之一;SESA 检测 AsAb 反应具有比 F-D 法更高的特异性、敏感性和精确性。
By means of Solid Enzyme Staining Assay(SESA)and Tube Slide Agglutination Test(F-D),the Antisperm Antibodies(AsAb)in the serum and cervical mucus of infertiledairy cows(experimental group)and heifers,normal open and pregnant cows(controlgroup)were detected.The results showed that average positive reaction rate of AsAb in theexperimental group detected by SESA(36.5%, n=170)was significantly higher than thatin the control(4.9%,n=41,P<0.001).The positive rate of AsAb in the cervical mucusof infertile cows(37.9%,n=29)was also higher than the control(0%,n=8,P<0.05).The titres of serum and cervical mucus reacted positively in AsAb were 1:133 and 1:141respectively(P>0.05).The results measured by F-D showed that positive rate of AsAb inthe experimental group was higher than that of heifers(P<0.05),although no remarkabledifference was found in comparision with the open and pregnant cows(P>0.05).The posi-tive reaction rate of AsAb was not related to service time and parity.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
1990年第3期81-86,共6页
Journal of Nanjing Agricultural University
关键词
牛
奶牛
精子
免疫学
不育症
antisperm antibody
serum
cows
infertility
