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Tetracysteine as a Reporter for Gene Therapy 被引量：1

Tetracysteine as a Reporter for Gene Therapy
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摘要 Objective To study the feasibility of using tetracysteine （TC） reporter in gene therapy. Methods Effects of TC reporter and conventional reporter genes encoding green fluorescence protein （GFP） and luciferase （Luc） on expression and function of the therapeutic gene MGMT^P140K were compared. Cytotoxicity and drug resistance were studied by Western blot. TC reporter used in therapy was analyzed by flow cytometry （FCM）. Results The TC reporter had no toxicity to cells and neither affected the expression or activity of therapeutic gene as compared to GFP and Luc. TC could be used in blood sample detection. Conclusion TC is a new kind of reporter gene for lentiviral vector in future gene therapy. Objective To study the feasibility of using tetracysteine （TC） reporter in gene therapy. Methods Effects of TC reporter and conventional reporter genes encoding green fluorescence protein （GFP） and luciferase （Luc） on expression and function of the therapeutic gene MGMT^P140K were compared. Cytotoxicity and drug resistance were studied by Western blot. TC reporter used in therapy was analyzed by flow cytometry （FCM）. Results The TC reporter had no toxicity to cells and neither affected the expression or activity of therapeutic gene as compared to GFP and Luc. TC could be used in blood sample detection. Conclusion TC is a new kind of reporter gene for lentiviral vector in future gene therapy.
作者 CHEN-YU XU YING GU WANG-HENG HOU YU-QIONG QUE SHUANG-GUAN GAO TONG CHENG NING-SHAO XI
机构地区 National Institute of Diagnostics and Vaccine Development for Infectious Diseases Key Laboratory of Ministry of Education for Cell Biology and Tumor Cell Engineering
出处 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2009年第6期496-501,共6页 生物医学与环境科学（英文版）
关键词 Tetracysteine Lentiviral vector REPORTER Gene therapy Tetracysteine Lentiviral vector Reporter Gene therapy
分类号 Q78 [生物学—分子生物学] G214.2 [文化科学—新闻学]
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参考文献29

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同被引文献14

  • 1Adam S. Cockrell,Tal Kafri.Gene delivery by lentivirus vectors[J]. Molecular Biotechnology . 2007 (3)
  • 2Shizuko Sei.Peptide nucleic acids as epigenetic inhibitors of HIV-1[J]. International Journal of Peptide Research and Therapeutics . 2003 (3-4)
  • 3Tang H,Kuhen KL,Flossie WS.Lentivirus replication andregulation. Annual Review of Genetics . 1999
  • 4Sung MH,Simon R.Genomewide conserved epitope profiles ofHIV-1 predicted by biophysical properties of MHC bindingpeptides. Journal of Computational Biology . 2004
  • 5Kock N,Kasmieh R,Weissleder R,et al.Tumor therapymediated by lentiviral expression of shBcl-2 and S-TRAIL. Neoplasia . 2007
  • 6Heldt SA,Ressler KJ.Amygdala-specific reduction of alpha1-GABAA receptors disrupts the anticonvulsant,locomotor,andsedative,but not anxiolytic,effects of benzodiazepines in mice. The Journal of Neuroscience . 2010
  • 7Wu Y,Beddall MH,Marsh JW.Rev-dependent lentiviralexpression vector. Retrovirology . 2007
  • 8Mautino MR.Lentiviral vectors for gene therapy of HIV-1 infection. Current Gene Therapy . 2002
  • 9Zhang,X,Kim,J,Ruthazer,R,McDevitt,MA,Wazer,DE,Paulson,KE,Yee,AS.The HBP1 transcriptional repressor participates in RAS-induced premature senescence. Molecular and Cellular Biology . 2006
  • 10Asparuhova MB,Barde I,Trono D,Schranz K,Schumperli D.Development and characterization of a triple combination gene therapy vector inhibiting HIV-1multiplication. The Journal of Genetic Medicine . 2008

引证文献1

Biomedical and Environmental Sciences
2009年 第6期

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