摘要
目的探讨检测大肠癌患者术中腹腔冲洗液CEA、GCC和CD44v6 mRNA表达的临床意义。方法收集60例大肠肿瘤患者的术中腹腔冲洗液,其中大肠癌48例(大肠癌组),大肠良性病变12例(阴性对照组),以上标本均经病理学证实,并以人大肠癌细胞株SW480作为阳性对照;应用基于b-DNA信号放大的基因表达定量检测技术测定腹腔冲洗液中脱落细胞的CEA、GCC和CD44v6 mRNA表达,同时做腹腔冲洗液细胞学检查(PLC),进而结合临床相关资料分析CEA、GCC和CD44v6 mRNA表达的临床意义。结果大肠癌组腹腔冲洗液CEA、GCC、CD44v6的表达阳性率分别为43.8%(21/48)、60.4%(29/48)、31.2%(15/48),总阳性率为72.9%(35/48),高于腹腔冲洗液PLC的阳性率4.2%(2/48),上述3项指标在人大肠癌细胞株SW480中表达均为阳性;在阴性对照组中有1例CEA mRNA检出阳性,其余均为阴性表达。不同Dukes分期、肿瘤分化程度、浆膜侵犯程度、是否淋巴结转移对CEA、GCC和CD44v6 mRNA的阳性率差异有统计学意义(P<0.05),不同肿瘤大小、患者年龄和性别差异无统计学意义(P>0.05)。结论检测腹腔冲洗液中CEA、GCC和CD44v6 mRNA的表达有助于大肠癌腹腔微转移的预测及术后治疗方案的制定;GCC、CD44v6可作为检测大肠癌腹腔脱落癌细胞的指标;基于b-DNA信号放大的基因表达定量检测技术是一种预测腹腔微转移的有效方法。
Objective To explore the significance of the transcripts of carcinoembryonic antigen （CEA）, guanylyl cyclase C （GCC） and metastasis-related gene CD44v6 in peritoneal lavages of colorectal cancer patients. Methods The peritoneal lavages of 48 patients with identified colorectal cancer （colorectal cancer group） and 12 patients with benign colorectal disease （negative control group） were collected. All of the patients were pathologically confirmed. Human colorectal cancer cell line SW480 were taken as a positive control. The mRNA levels of CEA, GCC and CD44v6 in the collected cells were detected by QuantiGene Reagent System, which is a quantitative RNA detection method based on branched DNA （b-DNA） signal amplification technique. Meanwhile, cytologic examination with microscopy was performed. Results The positive expression rates of CEA, GCC and CD44v6 in the peritoneal lavages were 43.8% （21/48）, 60.4% （29/48） and 31.3% （15/48） respectively, and the total positive rate was 72.9% （35/48） in colorectal cancer group, which was more sensitive than that of cytologic examination （2% , 1/48 ）. All 3 marker genes were expressed in human colorectal cancer cell line SW480. There was only 1 sample positive to CEA mRNA in the negative control group. The positive rate of CEA, GCC and CD44v6 expressions were correlated with the Dukes staging, differentiation status, serosal involvement and lymph node metastasis （ P 〈 0. 05 ）, but not with tumor size, patient age and gender （P 〉 0.05）. Conclusion The detection of CEA, GCC and CD44v6 mRNA in peritoneal lavages is helpful for early diagnosis, prognosis assessment and suitable treatment selection for colorectal carcinoma.Similar to CEA, GCC and CD44v6 may be used as indicators for peritoneal micrometastasis of colorectal cancer. The combined application of these 3 molecular markers will improve the positive detection rate of peritoneal micrometastasis. The b-DNA signal amplification teehnique-based mRNA deteetion is more sensitive and speeific than that of eytologie examination, and is an effective way to predict peritoneal mierometastasis.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2010年第1期36-40,共5页
Journal of Third Military Medical University
基金
四川省科技厅应用基础研究项目(04JY029-015-3)~~
