摘要
目的:观察不同条件下、不同浓度的PTH对发育期大鼠髁突软骨细胞增殖功能和髁突发育的影响。方法:消化法培养大鼠髁突软骨细胞,PTH不同作用方式下MTT法检测细胞增殖能力。24只SD大鼠,分别在其发育期于左侧髁突关节腔内注射不同浓度(1μg/kg、5μg/kg、10μg/kg)PTH,待发育期结束后,处死,取双侧髁突,通过大体、组织学观察髁突生长情况。结果:低浓度间断PTH培养组的髁突软骨细胞增殖功能明显大于持续作用组和对照组,以含PTH0.001μg/mL培养液间断作用组的细胞增殖功能最强,而含PTH0.01μg/mL培养液持续作用组的细胞增殖最弱。注射PTH侧髁突体积均略大于未注射侧、对照组和空白组。苏木精-伊红染色见各组髁突表面基本完整、光滑,层次清晰。结论:小剂量间断的PTH作用对髁突软骨细胞的增殖有促进作用,局部注射PTH对髁突发育是否有促进或抑制作用有待进一步研究。
Objective： To investigate the effect of the proliferation capability of mandibular condylar chondrocytes and the development of condyle process with PTH by using different modes of action in rats. Methods： Mandibular condylar chondrocytes of neogenesis SD rats were isolated with enzyme digestion and cultured in DMEM medium with PTH on different modes of action. The proliferation of cells to PTH was determined by MTT. PTH of differ ent concentrations （1μg/kg, 5μg/kg, 10μg/kg） were injected to the left condyle process joint capsules during the growth period of 24 SD rats. At the end of growth period both sides of the condyle process were observed to investigate the development of the condyle process. Results： Low concentration and interruptive use of PTH can enhance the proliferation of mandibular condylar chondrocytes, and PTH with the concentration of 0. 001μg/ml has the most significant effect on the proliferation of mandibular condylar ehondrocytes, while persistent injection of PTH with the concentration of 0.01μg/ml has the most weak proliferation capacity to the mandibular condylar chondrocytes. In animal models with different concentrations of PTH injected to the left side, it＇s found that the volume of condyle process of the injected side is a little bigger than that of the other side, and is also bigger than that of the control group. Through tissue sagittal plane section, it＇s found that the surface of the condyle process are integrated and slick with a clear layer. Conclusion： Low concentration and interruptive use of PTH can lead to a high proliferation of mandibular condylar chondrocytes, while the effect of the same concentration of PTH injected to the condyle process on mandible＇s development needs further investigation.
出处
《口腔医学研究》
CAS
CSCD
北大核心
2009年第6期709-712,共4页
Journal of Oral Science Research
基金
国家自然科学基金资助项目(编号:30672341)
关键词
甲状旁腺激素
髁突软骨细胞
下颌骨发育
细胞增殖
Parathyroid Hormone Mandibular condylar chondrocytes Mandible development Cell proliferation
