摘要
目的：研究帕金森病（ＰＤ）黑质多巴胺（ＤＡ）神经元的死亡机制。方法：用脱氧核糖核酸（ＤＮＡ）标记法，借助荧光显微镜观察多巴胺对ＰＣ１２细胞凋亡的影响。结果：ＤＡ可诱发ＰＣ１２细胞凋亡，适中浓度时（０．４５ｍｍｏｌ／Ｌ）ＰＣ１２细胞凋亡数最多（凋亡率为４８．７％±６．３％）；抗氧化剂Ｎ乙酰半胱氨酸（ＮＡＣ）可部分阻止ＤＡ诱发的ＰＣ１２细胞凋亡（Ｐ＜０．０１）。结论：凋亡参与了ＰＤ的病变过程。
Objectives:To study the mechanism of dopaminergic neuronal death in substantia nigra of Parkinson's diseaes(PD). Methods:The effect of dopamine(DA) on apoptotic PC12 cell were examined by fluorescence microscopy using the DNA end-labeling methods. Results:DA may induce apoptosis in PC 12 cell,moderate concentrations of DA(0.45mmol/L4) giving maximal number of apoptotic cell (rate of apoptosis:48.7%±6.3%);and the antioxidant N-acetyl-cysteine(NAC) partially prevent DA-induced apoptosis in PC12 cells(P>0.01). Conclusion:Apoptosis may be involved in the pathogenesis of PD, and treatment with proper antioxidants amy be useful in PD therapy.
出处
《上海医学》
CAS
CSCD
北大核心
1998年第9期514-515,共2页
Shanghai Medical Journal
