摘要
目的探讨原癌基因ｂｃｌ２与抑癌基因ｐ５３在大肠腺瘤和大肠癌中的表达变化与大肠腺瘤和大肠癌的发生、发展的关系，以及ｂｃｌ２与ｐ５３在大肠癌中的表达变化与大肠癌的临床病理特征间的关系．方法采用免疫组化ＡＢＣ法，分别检测ｂｃｌ２在３３例大肠腺瘤和４７例大肠癌中的表达变化和ｐ５３在４０例大肠腺瘤和６３例大肠癌中的表达变化．结果ｂｃｌ２在大肠腺瘤和大肠癌中的阳性表达分别为７２７％（２４／３３）和３４０％（１６／４７）．ｐ５３在大肠腺瘤和大肠癌中的阳性表达分别为４５０％（１８／４０）和８４１％（５３／６３），ｂｃｌ２和ｐ５３在大肠腺瘤和大肠癌中的表达有显著性差异（Ｐ＜００１），但其表达变化与大肠癌的临床病理特征关系不明显（Ｐ＞００５）．结论ｂｃｌ２表达在腺瘤向癌的演变过程中丢失，而ｐ５３表达则增加（Ｐ＜００１），表明它们参与了肿瘤生长和发展的共同通道，在大肠癌的发生。
AbstractAIM To investigate the relationship between protooncogene bcl-2 and suppressor oncogene p53 expressions, development, progress and clinical pathological features in colorectal adenoma and carcinoma.METHODS Expressions of oncogene bcl-2 were determined in 33 cases of colorectal adenomas and 47 cases of colorectal carcinomas by the immunohistochemical method (ABC method), and p53 in 40 cases of colorectal adenomas and 63 cases of colorectal carcinomas.RESULTS The positive expression rates of bcl-2 in colorectal adenomas and carcinomas were 72.7% (24/33) and 34.0% (16/47) respectively, and the positive staining rates of p53 were 45.0% (18/40) and 84.1% (53/63) respectively. There was significant difference in bcl-2 and p53 expressions between colorectal adenomas and carcinomas (P<0.01). But their expressions were not associated with clinical pathological features of colorectal carcinomas.CONCLUSION bcl-2 expression loses in the process from colorectal adenomas to carcinomas development, but p53 increases. bcl-2 and p53 take place in common pathways in tumor development and progression, and play an important role in colorectal carcinomas.
