摘要
目的：探讨ｖ－ｓｉｓ、ｃ－ｍｙｃ、ｃ－ｆｏｓ三种癌基因ｍＲＮＡ的协同表达在脑膜瘤中的作用。方法：用原位杂交结合图像分析方法对三种癌基因在脑膜瘤中的ｍＲ－ＮＡ表达状况进行了定量研究。结果：ｖ－ｓｉｓ、ｃ－ｍｙｃ、ｃ－ｆｏｓ在脑膜瘤中的表达率依次为６０．７％、４３．３％和５７．１％，脑膜瘤中ｃ－ｍｙｃ和ｃ－ｆｏｓ的ｍＲＮＡ表达间呈正相关，而ｖ－ｓｉｓ和ｃ－ｍｙｃ的ｍＲＮＡ表达间只在纤维细胞型脑膜瘤（ＦＭ）中呈正相关。结论：这三种癌基因表达增高在脑膜瘤中具有一定作用。ｃ－ｍｙｃ和ｃ－ｆｏｓ的协同表达在脑膜瘤发生发展中具有特殊意义，ｖ－ｓｉｓ和ｃ－ｍｙｃ协同表达在ＦＭ中也有特异性作用，说明各组织分型的脑膜瘤在发生发展中具有一定的遗传异质性。
Objective:To study the cooperation of vsis,cmyc and cfos in meningiomas.Methods:Using in situ hybridization and image analysis methods,we studied the mRNA expression of the three oncogenes in quantity.Results:The expression rates of vsis,cmyc and cfos were 607%,433% and 571% in turn.The mRNA expression between cmyc and cfos presented positive correlation in meningiomas,and so did the mRNA expression between vsis and cmyc only in fibroblastic meningiomas(FM).Conclusion: The overexpression of the three oncogenes plays a certain role in meningiomas.The cooperation expression of cmyc and cfos has special significance in pathogenesis and development of meningiomas,and the cooperation expression of vsis and cmyc has specific effect on FM,too.This also indicates that there is a certain genetic heterogeneity in initiation and development of all kinds of histological subtypes of meningiomas.
出处
《滨州医学院学报》
1998年第4期321-323,共3页
Journal of Binzhou Medical University
关键词
脑膜瘤
癌基因
协同表达
原位杂交
图像分析
meningiomas
oncogenes
cooperation expression,in situ hybridization
image analysis
