摘要
目的探讨不同的抗原修复方法对肾活检标本石蜡切片免疫荧光染色的影响。方法将20例肾活检标本分别制成石蜡切片(实验组)及冰冻切片(对照组),其中实验组石蜡切片分成应用胰酶抗原修复组;高压加热抗原修复组及无抗原修复组,后行免疫荧光染色,并与冰冻切片免疫荧光染色相比较。结果石蜡切片胰酶抗原修复组免疫荧光染色结果与冰冻切片免疫荧光染色结果基本符合;高压加热抗原修复组出现较多的假阴性结果;无抗原修复组染色效果较差;以上三组间存在统计学差异。结论石蜡切片胰酶抗原修复免疫荧光染色可以作为冰冻切片免疫荧光染色标本无肾小球时较好的补救措施。
Objective To investigate the effect to immunofluorescence dying using paraffin section,taking different means of antigen retrieval.The samples were from renal biopsy.Methods 20 samples of renal biopsy were taken frozen section and paraffin section.Then the paraffin sections of every sample were devided by three group.The first group used tryptic enzyme to take antigen retrieval.The second group used high-pressure heater to take antigen retrieval.The third group was no antigen retrieval.Then all the section took immunofluorescence dying.The three groups of paraffin sections compared with the frozen sections.Results The coincidence rate that the first group of paraffin section compared with the frozon sections was the best in the three groups of paraffin section.Conclusion Immunofluorescence dying using paraffin section which the means of antigen retrieval used tryptic enzyme was a better remark measures,when there were no glomerulus in the frozen section.
出处
《中国实验诊断学》
北大核心
2009年第12期1728-1730,共3页
Chinese Journal of Laboratory Diagnosis
关键词
肾活检
石蜡切片
免疫荧光染色
Renal biopsy
Paraffin section
Immunofluorescence dying
