摘要
目的构建pEGFP-N1/BMP-2真核表达质粒,并进行鉴定。方法利用RT-PCR方法从组织中提取骨形态发生蛋白(BMP-2)的基因片段,与pMD18-T载体连接,构建pMD18-T/BMP-2重组质粒,酶切后与pEGFP-N1真核表达载体连接,构建pEGFP-N1/BMP-2真核表达质粒,进行测序、酶切鉴定,表明质粒构建成功。结果通过测序、酶切鉴定,证明pMD18-T/BMP-2质粒构建成功。结论本实验成功构建了pEGFP-N1/BMP-2真核表达质粒,为进一步研究利用BMP-2基因修饰骨组织工程骨种子细胞提供实验基础。
Objective To construct a eukaryotic expression vector carrying BMP-2 gene.Methods BMP-2 gene was cloned from tissue by using RT-PCR method and was inserted into pMD18-T to constrcuct pMD18-T/BMP-2 plasmid,and to construct pEGFP-N1/BMP-2 plasmid by using restriction enzyme.To identify the plasmid by sequencing and restriction enzyme.Results The eukaryotic expression vector pEGFP-N1/BMP-2 was constructed and identified.Conclusion To construct the expression vector successfully,BMP-2 gene was able to was for bone engineering.
出处
《中国实验诊断学》
北大核心
2009年第12期1683-1685,共3页
Chinese Journal of Laboratory Diagnosis
基金
高等学校博士点专项科研基金(编号:20060183050)
