摘要
目的观察人乳头瘤病毒（ＨＰＶ）Ｅ６癌基因蛋白阳性食管鳞癌的超微结构特性，探讨食管鳞状细胞癌与Ｅ６癌基因蛋白的关系．方法采用微波免疫组化技术，对５３例食管鳞癌ＨＰＶ阳性病例进行ＨＰＶＥ６蛋白检测，同时进行电镜观察．结果４５例阳性的食管鳞癌中，高分化鳞癌２例（４４％），中分化鳞癌１８例（４００％），低分化鳞癌２５例（５５６％）．电镜观察：ＨＰＶ阳性的食管鳞癌主要呈低度分化．除显示核质比例增加，核外形不规则以外，核小体及染色质间、染色质周围颗粒显著增加．超微结构中未见病毒颗粒．结论食管鳞状细胞癌中ＨＰＶＥ６蛋白与食管癌细胞的异常增生、代谢旺盛有一定的关系．
IM To study the expression of HPV16E6 protein and ultrastructure in human esophageal cancer. METHODS By using immunohistochemical method, 45 patients with positive HPV16E6 protein were studied and microscopic observation was made. RESULTS Nuclear HPV16E6 protein was present in 45 (849%) cases of esophageal squamous cell cancer specimens in HPV DNA positive patients by immunohistochemical method. Under the electron microscope, the inter and perichromatin granules as well as nuclear bodies were increased in the nuclei in all biopsies with positive HPV16E6 protein, but no HPV particle was found in cancer cells. CONCLUSION HPV16DNAE6 was integrated into the cellular genome. The E6 early genes of HPV16DNA were closely related to the inter and perichromatin granules as well as nuclear bodies hyperplasia in the nuclei of esophageal cancer. .
关键词
癌基因蛋白质
食管肿瘤
超微结构
免疫组化
HPV
ncogene proteins, viral/metabolism
esophageal neoplasms/metabolism
carcinoma, squamous cell/metabolism
esophageal neoplasms/ultrastructure
carcinoma, squamous cell/ultrastructure
