摘要
目的探讨柞蚕免疫血淋巴（主要成分为柞蚕杀菌肽）对肿瘤细胞的杀伤及选择性杀伤作用机制．方法采用３ＨＴｄＲ掺入法及扫描、透射电子显微镜观察柞蚕免疫血淋巴对人直肠癌细胞的生长抑制、细胞ＤＮＡ合成及超微结构的影响．结果柞蚕免疫血淋巴在低浓度（１∶１６）即可抑制癌细胞生长及３ＨＴｄＲ掺入ＤＮＡ，随浓度增加，作用时间延长，作用逐渐增强；扫描、透射电子显微镜下见作用３０ｍｉｎ人直肠癌细胞超微结构即出现改变，２４ｈ～４８ｈ细胞膜结构破坏致细胞溃解．而作用于正常猴肾细胞ＣＶ１２４ｈ未见细胞生长及３ＨＴｄＲ掺入ＤＮＡ受到抑制；亦未见细胞超微结构破坏．结论柞蚕免疫血淋巴（柞蚕杀菌肽）对肿瘤细胞具有杀伤及选择性杀伤作用；主要作用部位在细胞的膜性结构；
IM To study the mechanism of the selective cytocidal effect of immunized hemolymph (main ingredient cecropins) from pupae of the Chinese oak silkworm, Antheraea pernyi (A. Pernyi) on cancer cells. METHODS The fine structure, inhibition of proliferation and synthesis of DNA of the rectal cancer cells induced by the immunized hemolymph were studied under electron microscopy and by the 3HTdR incorporation method. RESULTS The immunized hemolymph from pupae of A.Pernyi inhibited the proliferation of cells and incorporation of 3HTdR into HR8348 cells at a low concentration (1∶16), being time and concentrationdependent. The fine structures of HR8348 cells have changed after 30 minutes exposure. By 24-48 hours, the membranes of the cancer cells were damaged and the cells were lysed; but these damages could not be observed in normal mammal cells (CV1). CONCLUSION The immunized hemolymph can kill the cancer cells but not damage the mammal cells (selective cytocidal effect). The main acting site is the membranous structure. .
