摘要
目的探讨慢性丙型肝炎患者基线T细胞分化状态与抗病毒治疗快速病毒学应答(RVR)的关系。方法采用流式细胞仪检测CD27和CD28在CD8+T细胞上的表达,根据其表达差异,判断CD8+T细胞的不同分化状态:早期(CD28+/CD27+)、中期(CD28-/CD27+)和晚期(CD28-/CD27-);比较抗病毒治疗获得RVR组和非RVR组的基线CD27、CD28的表达水平及T细胞分化状态的差异。结果获得RVR组的CD27、CD28在CD8+T细胞的表达均高于未获得RVR组,差异有统计学意义(P=0.004,P=0.01)。T细胞的分化状态在RVR组以早、中期为主,而在非RVR组以晚期T细胞分化状态为主,2组之间差异有统计学意义(P<0.01)。结论基线T细胞的分化状态与干扰素联合利巴韦林抗病毒治疗慢性丙型肝炎RVR有一定相关性,可能与机体T细胞免疫在干扰素的免疫调节下参与病毒清除有关。
Objective To investigate the association between the differentiations of CD8＋ T cell before treatment and the rapid virological response to antiviral therapy in patients infected with hepatitis C virus（HCV）. Methods The presences of CD27 and CD28 on the surface of CD8＋ T-cell were monitored using flow cytometry in 40 patients chronically infected with HCV before treatment with pegylated alpha interferon and ribavirin. The differentiations of CD8＋T cell were assessed at early stage（CD28＋/CD27＋）,intermediate stage（CD28-/CD27＋） and late stage（CD28-/CD27-）. Then,a comparison on differentiations of CD8＋T cells between patients who presented a rapid virological response（RVR） to therapy and those who did not show any RVR（no RVR） was performed. Results A clear difference in the presences of CD27 and CD28 on the surface of CD8＋ T-cell was found：in patients with RVR,the percentages of CD27 and CD28 were much higher than those in patients without RVR,the difference was significant（P=0.004 and P=0.01,respectively）. Moreover,the differentiations of CD8＋T cells were compared between patients with RVR and without RVR：in patients with RVR,the dominant cells were the ones in early/intermediate stage,while,a late differentiation process of CD8 T cells might be associated with lack of rapid response to treatment. Conclusion The differentiations of CD8＋ T cell before treatment are related to the rapid virological response to antiviral therapy in patients infected with HCV,this phenomenon indicates that cytotoxic T cell may be involved in the clearance of HCV during the treatment with interferon.
出处
《首都医科大学学报》
CAS
北大核心
2009年第6期775-779,共5页
Journal of Capital Medical University
基金
国家"十一五"科技重大专项(2008ZX10002-013)资助项目~~
