摘要
目的探讨西地那非对糖尿病大鼠肾脏的保护作用及机制。方法将28只雄性SD大鼠分为正常对照组、正常＋西地那非处理组、糖尿病组、两地那非治疗组，每组7照。药物十预8周后，检测各组大鼠的血糖（BG）、24h尿白蛋白量、肌酐水平。随后处死大鼠．测定肾组织中总抗氧化能力（T—AOC）、丙二醛（MDA）含量、谷胱甘肽过氧化酶（GSHPX）活性。同时留取肾组织作HE染色行病理检查。RTPCR法检测肾组织中单核细胞趋化蛋白－1（MCP－1）mRNA表达。结果与糖尿病组相比，西地那非治疗组BG无统计学差异，而24h尿内蛋白量、Ccr、MDA、MCP1mRNA表达牡著下降，T-AOC、GSHPX活性上升，差异具有统计学意义，肾组织病理改变减轻。结论西地那非可以改善糖尿病大鼠肾脏损害，其机制可能与其抗氧化、降低MCP-1表达有关。
Objective To investigate the protective effects of sildenafii oil the kidney of diabetic rats and the mechanism. Methods 28 male SD rats were equally divided into four groups： normal rats,normal rats treated with sildenafil, diabetic rats, diabetic rats treated with sildenafil. After 8 weeks, blood glucose, blood creatinine and 24 h urinary albumin were measured. 1.ipid peroxidatin, ma londialdehye（MDA）, glutathione peroxidase （GSH-PX） and total antioxidative capacity （T-AOC） in the renal tissues were determined. The renal tissue was cxamined by light microscopy. The expression levels of monocyte chemoattractant protein I （MCP-1） mRNA were semi-quantitatively detected by reverse transcription-polymerase chain reaction. Results No significant differences in blood glucose were found between diabetic group and sildenafil treated groups. As compared with diabetic group, the levels of Ccr,24-h urinary albumin, renal MDA, MCP 1 mRNA were decreased significantly, but the activity of renal GSH PX and T-AOC increased significantly in sildenafil-treated groups. Renal pathologic changes in sildenafil-treated group were also alleviated. Conclusion Sildenafil could alleviate the renal pathologic changes in diabetic rats, which is closely related to the antioxidization and the decrease of MCP-1 activity in renal tissue.
出处
《临床肾脏病杂志》
2009年第12期558-561,F0003,共5页
Journal Of Clinical Nephrology
关键词
大鼠
糖尿病肾病
氧化性应激
丙二醛
Rats
Diabetic kidney diseases
Oxidative stress
Malondialdehyde
