摘要
以葡萄孢属TB-3菌株为出发菌株制备其原生质体．在纤维素酶浓度为20g／L，蜗牛酶浓度为3g／L的酶解系统中，25℃酶解3h，其原生质体制备数可达67×105mL-1，在KYM上的再生率为46．1％，在KPDA上的再生率为33．6％，经过3轮原生质体紫外线诱变后回复再生，及对大量再生突变株进行发酵筛选，获得高产稳定株TB－3H8，其发酵液中ABA的质量浓度可达1．
Botrytis strain TB-3 was isolated and used as a starting strain to prepare its pn,toplasts under the conditions including cellulase at 20 g/L, snailase at 3 g/L and incubation at 25℃ for 3 h, resulting in the protoplast density Of 6. 7 x 10 5 mL-1. The protoplasts were mutated by UV irradiation for 3. 5 min and the normal fungal colonies were regenerated then with freqUencies of 46. 1 % and 33. 6% on KYM and KPDA,respectively. After three cycles of selection procedures including protoplast UV irradiation, colony regeneration,primary and secondary fermentation screening, a high-yield and genetically stable strain TB-3-H8 was obtained.The ABA yield was increased from 0.2 g/L by the starting strain to 1.4 g/L by TB-3-H8.
出处
《应用与环境生物学报》
CAS
CSCD
1998年第3期281-285,共5页
Chinese Journal of Applied and Environmental Biology
基金
国家自然科学基金!39400003
中科院成都地奥科学基金
