摘要
从南洋楹新鲜根瘤中分离得到根瘤菌菌株8638，再经稀释分离，挑取不同形态的菌落，经连续单菌落纯化，获得8638L、8638M和86385三株菌落型．对它们的形态特征、培养性状、生长速度、生理生化特性进行观测．并对它们回接南洋楹后的结瘤状况和共生固氮活性，以及在自生条件下固氮酶和吸氢酶活性作了测定．
The Rhizobium sp. strains 8638L, 8638M and 86385 were isolated from root nodules of Albizia falcataria which were Gram-negative rods with a subpolar flagellum, the cell sizes being 0.53 ×1 .17, 0.66 ×1.95 and 0.66 × 2.03 up, respectively. The growth of the three strains colonies in yeast-mannitol (YM) liquid medium had long doubling time (7.79, 10.16 and 8.7 h, respectively). They grew slowly on YM agar, and could produce alkali, but could not grow on medium with 5% NaCl and at 39 ℃. Physiological and biochedrical characteristics of the three rhizobial strains were examined. The nodulation and nitrogenase activity in seedlings of A. falcataria inoculated with the strains isolated from nodules of the same tree species were tested, which provided satisfactory results. The strains were shown to have nitrogen fixing ability under self-growing condition.
出处
《热带亚热带植物学报》
CAS
CSCD
1998年第3期216-220,共5页
Journal of Tropical and Subtropical Botany
关键词
南洋楹
根瘤菌
特性
Albizia falcataria, Rhizobial strains, Characterization
