摘要
用双重免疫染色包埋前免疫电镜法，在光镜和电镜水平观察了大鼠孤束核内降钙素基因相关肽神经元的分布及其神经末梢与儿茶酚胺能神经元之间的关系。降钙素基因相关肽阳性神经元呈棕色，主要分布于孤束核的连合亚核、内侧亚核以及背侧周边区。在孤束核的尾侧部、连合亚核的腹外侧部及背侧周边区观察到蓝绿色的酪氨酸羟化酶阳性神经元和棕色的降钙素基因相关肽阳性神经元和神经纤维，其中一些神经元内两者共存。电镜观察结果：酪氨酸羟化酶阳性神经元细胞质内有棒状或卵圆形的电子密度较高的吲哚-β-半乳糖苷酶反应产物，降钙素基因相关肽阳性神经末梢内见到电子密度较高的DAB反应产物以及清亮小泡和颗粒小泡。降钙素基因相关肽阳性神经末梢和酪氨酸羟化酶阳性神经元之间形成对称性轴一体突触。
In this paper the distribution of CGRP neurons and the relationship between CGRP nerve terminals and catecholaminergic neurons in the nucleus tractus solitarii of the rat was studied at light microscopic and electron microscopic levels, using double-immunostaining method and immunoelectronmicroscopic staining techniques. CGRP-positive neurons were stained browu and mainly distributed in the commissural subnucleus, medial subnucleus and dorsal peripheral areas of nucleus tractus solitarii. In the caudal part of nucleus tractus solitarii, ventrolateral region of the commissural subnucleus and dosal peripheral areas, bluegreen TH-positive neurons and brown CGRP-positive neurons and nerve fibers could be observed. In some of the neurons CGRP and TH were co-existed. Electron microscopy study: there were claviform or oviform electron-dense higher indole-β-galactosidase reaction products in the TH-positive neurons. In the CGRP nerve terminals, electron-dense higher DAB reactive products,clear vesicles and granular vesicles could be found. CGRP-positive nerve terminals and TH-positive neurons formed symmetrical axosomatic synapse.
出处
《神经解剖学杂志》
CSCD
北大核心
1998年第3期271-274,共4页
Chinese Journal of Neuroanatomy
