摘要
用Ｓｅｐｈａｒｏｓｅ－６Ｂ凝胶层析法纯化原代地鼠肾细胞狂犬病苗，可去除绝大部分杂蛋白，总蛋白含量减少９９．４％以上，纯化后抗原比活性提高２１０倍，单位疫苗剂量中牛血清含量降低１８．７倍以上。方法简单，价廉。
After twenty folods concentrated,Human rabies vaccine prepared from primary hamster kidney cell was purified on a sepharose6B agrose chromatography columm,The final product was purified to be 212-folds,contain of 14.5μg/IU of protein and no more than 1.94ng/IU of fetal calf serum.The recovery was aboout 100%.This method can be used in large-scale production.
出处
《微生物学免疫学进展》
1998年第3期18-20,共3页
Progress In Microbiology and Immunology
关键词
柱层析
地鼠肾细胞
狂犬病疫苗
Chromatography columm Rabies vaccine Primary hamster kidney cell
