摘要
采用激光和微波两种物理因子，对去甲基金霉素生产菌金霉素链霉菌进行诱变处理，选育到一株产量较高的生产菌ＨＬ１１，发酵效价从２８３１ｕ／ｍｌ提高到４６８３ｕ／ｍｌ，提高了６５４％。所选育的菌株经多次传代，遗传性状非常稳定。
A Mutant strain HL11 of high yielding demethychlorotelracycline was screened form its parent strain S.aureofaciens MHO1 by combining laser irradiation with microwave irradiation.The results showed that the titer of demethylchlorotetracycline of S.aureofaciens was increased from 2831 u/ml upto 4683 u/ml and the increasing rate was 65.4%.It's high capacity for producing demethychorotetracycline remained stable after many times of subculture and two months storage.
出处
《生物工程学报》
CAS
CSCD
北大核心
1998年第4期445-448,共4页
Chinese Journal of Biotechnology
关键词
激光诱变
微波诱变
金霉素链霉菌
去甲基金霉素
Microwave irradiation, laser irradiation, Streptomyces aureofaciens ,demethylchorotetracycline
