摘要
分别以Ｇ２００及其旁侧区的Ｙ２５８７Ｌ、Ｙ４０７３Ｌ和Ｌ６８８片段为探针筛选水稻基因组ＹＡＣ文库，共得到４个阳性克隆，均与Ｇ２００、Ｙ２５８７Ｌ和Ｙ４０７３Ｌ座位重叠，插入片段的大小为２４０～６５０ｋｂ。用反向ＰＣＲ法分离ＹＡＣ克隆插入片段的末端序列，并利用这些末端序列确定克隆的方向以及进行染色体步查，共筛选到７个ＹＡＣ克隆，建立了一个８厘摩（ｃＭ）的Ｇ２００ＹＡＣ重叠群。
A YAC contig was constructed by screening a rice genomic YAC library with G200,Y2587L,Y4073L and L688 RFLP markers as probes.4 positive clones with the sizes ranged from 240kb to 650kb were obtained.The end fragments of YAC inserts were isolated via inverse PCR,with whom a YAC contig of 3cM was initially established.Chromosome walking was performed with the two distal end fragments of the YAC contig and some positive YAC clones were obtained and identified. Thus the G200 contig was extended to about 8cM.
出处
《生物工程学报》
CAS
CSCD
北大核心
1998年第4期366-371,共6页
Chinese Journal of Biotechnology
基金
国家863计划
美国洛氏基金会水稻生物技术国际合作项目
